Library Detail Page for Ca_CatYoLeaf2

Short Name:Ca_CatYoLeaf2
Organism:Coffea arabica
Library Name: Coffee leaves PCRScript library
Total Sequences: 435 sequences from 448 clones
Average Sequence Length:411.2 (Standard deviation 155.2)
Type:cDNA Library
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CommentsOrgan: Leaf, Vector: PCRScript, The cDNA library was constructed from mRNA isolated from young coffee leaves. Complementary DNA was synthesized from mRNA using the primer oligo-dT/CDSIII (3' priming) and smart oligonucleotide that attaches at the 5' end during elongation with reverse transcriptase. The library is non-directional. Cycle sequencing of individual clones was performed and the samples run on an ABI377 sequencing machine. The project was a collaborative research initiative between the Colombian National Center for Coffee Research - CENICAFE and the laboratory directed by Dr. Susan McCouch, Department of Plant Breeding, Cornell University, Ithaca, NY. This research was partially financed by COLCIENCIAS-Instituto Colombiano para el Desarrollo de la Ciencia
AuthorsCristancho,M.A. and McCouch,S.R.
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