Library Detail Page for LeHRU

Short Name:LeHRU
Organism:Solanum lycopersicum (formerly Lycopersicon esculentum)
Library Name: Tomato root subtractive cDNA library for heat-shock up-regulated genes
Total Sequences: 297 sequences from 312 clones
Average Sequence Length:579.2 (Standard deviation 171.2)
Type:cDNA Library
Development Stage:Three weeks old
Treatment Conditions:
Cloning Host:DH5a
Cloning Kit:
CommentsVector: pGEM-Teasy, The subtractive library was created by using mRNA purified from tomato roots with or without heat-shock treatment. Tomato roots were harvested from plants of 3 weeks old seedlings grown on MS medium. Plants were heat-treated by incubated at 40 degree C in the dark for 4 h followed by recovery at room temperature for 1 hour. Library of up-regulated cDNA was generated by subtracting the heat-treated mRNA with non-treated ones.
AuthorsLiu,N.Y., Ko,S.S., Yeh,K.C., and Charng,Y.Y.
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