Library Detail Page for LePS

Short Name:LePS
Organism:Solanum lycopersicum (formerly Lycopersicon esculentum)
Library Name: Subtractive cDNA library of roots under phosphate starvation
Total Sequences: 817 sequences from 823 clones
Average Sequence Length:499.1 (Standard deviation 183.8)
Type:cDNA Library
Development Stage:5 weeks old plants
Treatment Conditions:
Cloning Host:Top 10 F'
Cloning Kit:
CommentsVector: PGEM Teasy, Tomato plants were grown hydroponically in modified Hoagland's solution containing 0.5 mM of KH2PO4. 4-week-old plants were subjected for phosphate starvation treatment by omitting the KH2PO4 from the nutrient solution. Tomato roots were harvested after 7-day phosphate starvation. Total and poly A+ RNA was isolated from phosphate starvation and control roots, respectively. Subtractive cDNA library was constructed using PCR-Select cDNA subtraction Kit (Clontech) to selectively amplify up-regulated genes under phosphate starvation condition.
AuthorsLiu,N.Y., Ko,S.S., Yeh,K.C., Charng,Y.Y., Shieh,S.Y., Wang,J.G., Lin,S.I., Cheng,P.W. and Chiou,T.J
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