Library Detail Page for LePU

Short Name:LePU
Organism:Solanum lycopersicum (formerly Lycopersicon esculentum)
Library Name: Normalized cDNA library from ripening tomato pericarp
Total Sequences: 1495 sequences from 1542 clones
Average Sequence Length:498.4 (Standard deviation 137.9)
Type:cDNA Library
Development Stage:Turning stage of fruit ripening.
Treatment Conditions:
Cloning Host:
Cloning Kit:
CommentsEqual amounts of total RNA isolated from two tomato genotypes differing in juice Bostwick value were mixed and used to prepare a normalized cDNA library (Takahashi-N and Ko-MSH, 1994: Toward a whole cDNA catalog: construction of an equalized cDNA library from mouse embryos. Genomics-San-Diego, 23: 1, 202-210). The resultant PCR amplified products were ligated into pBluscript SKII- vector (Stratagene) and transformed into E. coli DH5alpha
AuthorsSrivastava,A., Datsenka,T., Kudla,K., Bhaskaran,A. and Handa,A.K.
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