Library Detail Page for LeTrich01

Short Name:LeTrich01
Organism:Solanum lycopersicum (formerly Lycopersicon esculentum)
Library Name: Solanum lycopersicum trichomes
Total Sequences: 7159 sequences from 7299 clones
Average Sequence Length:561.4 (Standard deviation 189.2)
Type:cDNA Library
Tissue:Isolated leaf trichomes
Development Stage:Apical internodes of 5-week old plants.
Treatment Conditions:
Cloning Host:
Cloning Kit:
CommentsVector: pDNR_lib (Clontech), Site_1: Sfi I A (GGCCATTACGGCC), Site_2: Sfi I B (GGCCGCCTCGGCC), Solanum lycopersicum plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from the most apical leaves of 5 week old Solanum lycopersicum plants. Trichomes were harvested by brushing leaves frozen using liquid nitrogen and separated from contaminating leaf fragments by passing the frozen material through a sieve. RNA was isolated from the trichomes using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector.
AuthorsBesser,K., Harper,A., Welsby,N.A., Schauvinhold,I., Slocombe,S.P., Li,Y., Dixon,R.A. and Broun,P.
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