Library Detail Page for Petunia-C2H4
| Short Name: | Petunia-C2H4 |
| Organism: | Petunia hybrida |
| Library Name: | Petunia Floral Post Ethylene |
| Total Sequences: | 2452 sequences from 3072 clones |
| Average Sequence Length: | 462.7 (Standard deviation 114.6) |
| Type: | cDNA Library |
| Tissue: | all floral organs |
| Development Stage: | anthesis |
| Treatment Conditions: | Flowers at anthesis stage were excised from plants grown in standard greenhouses and treated with 2ppm exogenous ethylene. Entire flowers were collected at 0, 5, 10, 15, 24, and 36 hours after treatment. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis. |
| Cloning Host: | lambda ZAPII unidirectional |
| Cloning Kit: | Stratagene |
| Comments | |
| Authors | Kenichi Shibuya, Beverly Underwood, Holly Loucas, William Farmerie, Michelle Jones, David Clark* |
| Contact Information | David G. Clark\ University of Florida\ Environmental Horticulture Dept.\ 1545 Fifield Hall\ Gainesville, FL 32611-0670\ ph: 352-392-1831 x370\ FAX: 352-392-3870\ e-mail: geranium@ufl.edu\ http://hort.ifas.ufl.edu/floriculture/floriculture_bio.htm |
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