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Library Detail Page for Petunia-DevA
Petunia Floral Development
2095 sequences from 2870 clones
Average Sequence Length:
489.8 (Standard deviation 123.4)
all floral organs
Ten entire flowers of six developmental stages were collected on the same day from plants grown in standard greenhouses. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis. The flower stages were as follows in chronological order from youngest to oldest:\
stage 1 - no color in corolla; corolla 0.5 inches long\
stage 2 - first sign of color in corolla; corolla .75-1 inches long)\
stage 3 - fully elongated corolla (not open); corolla 1.5 inches long\
stage 4 - fully open corolla; anthers not yet dehisced\
stage 5 - fully open corolla; freshly anthesed, bright yellow pollen; wet stigma\
stage 6 - pre-senescent; yellowing of corolla tube; dry brown pollen (if present); stigma dry
lambda ZAPII unidirectional
Kenichi Shibuya, Beverly Underwood, Holly Loucas, William Farmerie, Michelle Jones, and David Clark*
David G. Clark\
University of Florida\
Environmental Horticulture Dept.\
1545 Fifield Hall\
Gainesville, FL 32611-0670\
ph: 352-392-1831 x370\
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