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Library Detail Page for Petunia-RF

Short Name:Petunia-RF
Organism:Petunia hybrida
Library Name: Petunia Fruit Ripening
Total Sequences: 687 sequences from 853 clones
Average Sequence Length:535.4 (Standard deviation 121.8)
Type:cDNA Library
Tissue:whole fruit (ovaries)
Development Stage:several
Treatment Conditions:Flowers at anthesis stage were self-pollinated and entire fruit (ovaries) were collected at 14, 16, 18, 20, 22, 24, and 26 days after pollination from plants grown in standard greenhouses. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.
Cloning Host:lambda ZAPII
Cloning Kit: Stratagene
Comments
AuthorsKenichi Shibuya, Beverly Underwood, Holly Loucas, William Farmerie, Michelle Jones, and David Clark*
Contact InformationDavid G. Clark\ University of Florida\ Environmental Horticulture Dept.\ 1545 Fifield Hall\ Gainesville, FL 32611-0670\ ph: 352-392-1831 x370\ FAX: 352-392-3870\ e-mail: geranium@ufl.edu\ http://hort.ifas.ufl.edu/floriculture/floriculture_bio.htm