Library Detail Page for SLMW
Short Name: | SLMW |
Organism: | Solanum tuberosum |
Library Name: | Low Molecular Weight |
Total Sequences: | 1135 sequences from 1368 clones |
Average Sequence Length: | 376.0 (Standard deviation 93.4) |
Type: | cDNA Library |
Tissue: | Assorted Stolon/Tuber Developmental Series |
Development Stage: | |
Treatment Conditions: | supplier: Developmental series. Plants from pathogen-free \ Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Individual stolon, swollen stolon, tuber skin and mature tubers (stored for one month at 9oC, 95% relative humidity), were collected and used for individual cDNA library syntheses. The cDNA purification column end fractions for each of these individual libraries were pooled and size-fractionated on an agarose gel. cDNAs between 100 bp and just under 500 bp were excised, purified and ligated into the cloning vector. \ |
Cloning Host: | XL10-Gold |
Cloning Kit: | Stratagene pBluescript II XR cDNA Library Construction Kit |
Comments | |
Authors | Flinn, B., Rothwell, C., Sardana, R., Griffiths, R.,\ Lague, M., De Koeyer, D., Audy, P., Goyer, C., Li, X-Q., Wang-Pruski, G., Regan, S.\ |
Contact Information | Barry Flinn\ bflinn@bioatlantech.nb.ca\ The Canadian Potato Genome Project - BioAtlantech Unit\ 921 College Hill Rd, Fredericton, ON, E3B 6Z9, CANADA\ |