Library Detail Page for TUBS
| Short Name: | TUBS |
| Organism: | Solanum tuberosum |
| Library Name: | Developing Tubers |
| Total Sequences: | 5111 sequences from 5976 clones |
| Average Sequence Length: | 481.2 (Standard deviation 168.1) |
| Type: | cDNA Library |
| Tissue: | Tubers |
| Development Stage: | |
| Treatment Conditions: | Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Developing tubers of 10-15 mm in length and 30-40 mm in length were collected early during the growing season and pooled. Mature tubers were collected\ approximately 1 3/4 months later at top kill, and then collected two weeks later post-top kill and pooled. RNA was isolated from the 2 pooled collections and mixed for library construction. A normalized library was constructed \ following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806). |
| Cloning Host: | XL10-Gold |
| Cloning Kit: | Stratagene pBluescript II XR cDNA Library Construction Kit |
| Comments | |
| Authors | Flinn, B., Rothwell, C., Sardana, R., Griffiths, R.,\ Lague, M., De Koeyer, D., Audy, P., Goyer, C., Li, X-Q., Wang-Pruski, G., Regan, S.\ |
| Contact Information | Barry Flinn\ bflinn@bioatlantech.nb.ca\ The Canadian Potato Genome Project - BioAtlantech Unit\ 921 College Hill Rd, Fredericton, ON, E3B 6Z9, CANADA\ |
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