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Library Detail Page for TUBS
5111 sequences from 5976 clones
Average Sequence Length:
481.2 (Standard deviation 168.1)
Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Developing tubers of 10-15 mm in length and 30-40 mm in length were collected early during the growing season and pooled. Mature tubers were collected\
approximately 1 3/4 months later at top kill, and then collected two weeks later post-top kill and pooled. RNA was isolated from the 2 pooled collections and mixed for library construction. A normalized library was constructed \
following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806).
Stratagene pBluescript II XR cDNA Library Construction Kit
Flinn, B., Rothwell, C., Sardana, R., Griffiths, R.,\
Lague, M., De Koeyer, D., Audy, P., Goyer, C., Li, X-Q., Wang-Pruski, G., Regan, S.\
The Canadian Potato Genome Project - BioAtlantech Unit\
921 College Hill Rd, Fredericton, ON, E3B 6Z9, CANADA\
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