Library Detail Page for cLEE

Short Name:cLEE
Organism:Solanum lycopersicum (formerly Lycopersicon esculentum)
Library Name: S.lycopersicum TA496 seeds and mature carpels
Total Sequences: 590 sequences from 547 clones
Average Sequence Length:459.9 (Standard deviation 111.8)
Type:cDNA Library
Tissue:seeds and maturing carpels
Development Stage:5 days post-anthesis to fruit over-ripe stage
Treatment Conditions:
Cloning Host:SOLR
Cloning Kit:
CommentsThe tissue for this library is from seed and maturing carpels. Fruit from 5 dpa, 10 dpa, 20 dpa, mature green, breaker, firm red ripe, and very soft red ripe have been collected and continue to be collected (since we will try subtraction/normalization with these). Seed has been removed from all stages, though there is relatively little from the 5 dpa stage. It have been combined 0.1 g of 5 dpa seed with 0.3g of 10 dpa, 0.7g of 20 dpa, and 0.3g from each of the mature green through late ripening stage seeds (1.2 g total for these 4) for RNA extraction and have enough poly A to give this a shot - about 5 ug.
AuthorsAlcala,J., Vrebalov,J., White,R. and Giovannoni,J.
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