Library Detail Page for cLEE
Short Name: | cLEE |
Organism: | Solanum lycopersicum (formerly Lycopersicon esculentum) |
Library Name: | S.lycopersicum TA496 seeds and mature carpels |
Total Sequences: | 590 sequences from 547 clones |
Average Sequence Length: | 459.9 (Standard deviation 111.8) |
Type: | cDNA Library |
Tissue: | seeds and maturing carpels |
Development Stage: | 5 days post-anthesis to fruit over-ripe stage |
Treatment Conditions: | |
Cloning Host: | SOLR |
Cloning Kit: | |
Comments | The tissue for this library is from seed and maturing carpels. Fruit from 5 dpa, 10 dpa, 20 dpa, mature green, breaker, firm red ripe, and very soft red ripe have been collected and continue to be collected (since we will try subtraction/normalization with these). Seed has been removed from all stages, though there is relatively little from the 5 dpa stage. It have been combined 0.1 g of 5 dpa seed with 0.3g of 10 dpa, 0.7g of 20 dpa, and 0.3g from each of the mature green through late ripening stage seeds (1.2 g total for these 4) for RNA extraction and have enough poly A to give this a shot - about 5 ug. |
Authors | Alcala,J., Vrebalov,J., White,R. and Giovannoni,J. |
Contact Information |