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Library Detail Page for cSTA

Short Name:cSTA
Organism:Solanum tuberosum
Library Name: Swelling Stolons
Total Sequences: 9803 sequences from 10537 clones
Average Sequence Length:481.1 (Standard deviation 109.2)
Type:cDNA Library
Tissue:Axillary buds of stemp explants; swelling stolons; growing stolons; growing sink-tubers
Development Stage:1 to 3 days (plates 1-20), 4 to 6 days (plates 21-40), 7 to 10 days (plates 41-60)
Treatment Conditions:tuber growth was induced or initiated in vitro as described by Bachem et al. (Plant Journal, 1996) Small microtubers develop from axillary bugs attached to stem explants when placed on a high sucrose medium (10%). Visible morphological changes occur synchronously at day five in the axillary buds.
Cloning Host:E.coli SOLR
Cloning Kit:
CommentsTissue was supplied by Christian Bachem and Richard Visser, Department of Plant Breeding, Wageningen University, The Netherlands. This library combines three seperate libraries: plates 1-20 consists of axillary bugs harvested on days 1-3. Plates 21-40 from days 4-6 in vitro tuberization. Plates 40-60 from day 7, 8, and 10 in vitro tuberization.
AuthorsRutger van der Hoeven, Julie Bezzerides, Christian Bachem, Richard Visser, and Steve Tanksley
Contact Information