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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ae423356f91511d984d7000bdb7dd3baseqwith50using36cmcapBRC_std
Basecaller-3730POP7SR.bcpDT3730POP7{BDv3}.mobtruefalsefalse20.00DT3730POP7{BDv3}.mobQUJJRgBldGRpcgAAAAED/wAcAAAACgAAA4AAAAGaAAAAAP//////////////////////////////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000.00falsefalse20false510false800false25.0falsefalsefalsefalse20true5502042020420falseBasecaller-3730POP7SR.bcpBasecaller {
#
# Last modified 08/10/2002
#
# ---------------------------
# SeqA Override options
# ---------------------------
defaultStart=1000
useDefaultStartAlways=0
defaultSpacing=12
useDefaultSpacingAlways=0
# ---------------------------
# Instrument/Run parameters
# ---------------------------
MODLOverride=3730
applyDownSampling=0
sampleRateOverride=0
sheathFlowCorrection=0
multicomponent=0
spacingCurveID=3
# ---------------------------
# Spacing Curve Model
# ---------------------------
spacingCalibration=12.2
cutoff1=2000.0
m1=0.0012
b1=10.0
cutoff2=5000.0
m2=0.0004
cutoff3=10000.0
m3=-0.0004
# ---------------------------
# Algorithm Parameters
# ---------------------------
normalizePeakHeights=3
optimizeSpacing=1
classifierType=1
medianFilterType=1
base1ID=1
deconvolution=0
fixNegativeRawData=1
clipBaselineToZero=1
}
-0.000410.00040.00120101000010.001301212.230101373010000.05000.02000.01
seqwith50using36cmcap
seqwith50using36cmcap2StdSeq36_POP71DC_Cap96_Fill14000DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability3.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4900BRC_long Run_BRC3730G_2005-07-20_08-05_0028 DNAservicesSeq 104587 R9_7-19-2005
76_571054_DT3730POP7{BDv3}.mobBRC_stdAPFN Z8 APXV 2 APrN %6 APrV 1 APrX #6 #6 %> BufT P| CTID Ph CTNM
Po CTTL $ CpEP DATA ? J DATA ? J DATA ? J < DATA ? J ~ DATA DATA l DATA DATA DSam DySN $ Dye# DyeN $ DyeN $ DyeN $ DyeN $ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
$l EVNT $x EVNT $ EVNT $ FWO_ GATC GTyp ! ! $ InSc InVt LANE % LIMS ! ! $ LNTD $ LsrP a MCHN MODF % MODL 3730 NAVG NLNE 0 OFFS PDMF Z# PXLB RGNm PY RMXV 1 RMdN Ht RMdV 2 RMdX H RPrN P- RPrV 2 RUND
RUND
RUND
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RUNT 6# RUNT ' RUNT
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SMLt SMPL Z SVER 2.0 SVER c SVER , , $@ ScSt Scal @ Scan ? TUBE D10 Tmpr < User Tom
BC 1.5.1.0AEPt ? AEPt ? APFN Z8 APXV 2 APrN %6 APrV 1 APrX #6 #6 %> ASPF ASPt ASPt BufT P| CTID Ph CTNM
Po CTTL $ CpEP DATA ? J DATA ? J DATA ? J < DATA ? J ~ DATA DATA l DATA DATA DATA 2g d 5 DSam DySN $ Dye# DyeN $ DyeN $ DyeN $ DyeN $ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
$l EVNT $x EVNT $ EVNT $ FWO_ GATC GTyp ! ! $ InSc InVt LANE % LIMS ! ! $ LNTD $ LsrP a MCHN MODF % MODL 3730 NAVG NLNE 0 OFFS PBAS R R m PBAS R R ~ PCON R R z PCON R R PDMF Z# PLOC R q PLOC R ? PXLB RGNm PY RMXV 1 RMdN Ht RMdV 2 RMdX H RPrN P- RPrV 2 RUND
RUND
RUND
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RUNT 6# RUNT ' RUNT
RUNT ' Rate $ RevC RunN # # P6 SCAN ? SMED
SMLt SMPL Z SPAC AW SPAC z SPAC AW SVER 2.0 SVER c SVER , , $@ ScSt Scal @ Scan ? TUBE D10 Tmpr < User Tom AGNCCNGTCATTGACACAAACTCTATCCCATGCTCAGTATCGACGACGTCTCCGCACTGCAACTGCTGCACGAATTCAGAAGGGTTTGATTCGGTACCTCTACATTATCATTGATTTCTCCAGGGTATGTAAAATCCTATATGATTGCAAAATAACTGCAGTATTGTAATTGGTTACTGACATTTAAAGGTTTAATTTTATTTTTTTTTGCAAAATATGAAACACTTATTTAAGCATCTATAGTTATCTGTTTGTAAATGAGTTATCTCCGGAAGAAGTTAATCAGGTTCAATTTCCCAGACAATCTGCTGATCTTCTTCTGATGCTAGCAAATTGTTTCTTAAGAAGGAATACAACAGGGTCCTTTTTAAAGAACCAGCAGCTCTTCGTTTTATGGATTTCTATTTGCATCTAGGGCTGAAGATTTAGTGATTGCAACTGGTTGATTATGCTATGAGATATTGGTAAAACTAAGCAATTGGGTCGTCTTTGATCAGTTTAATGAATATTCAAGTGAATACCTACTTTCTGTTTTGATGTCACCATATTTCTTTAAACCACGTTTTGAAATTCTAAAATTGTGAATTGAGTTTATAAGACAATTATTGCATTTTGAAAATGTCCCTTAATGGGGTTGTGCTTATCACTGCATTGGACGTTTTCTTTTATCATCGACAAGGTGCTTTAACAGACAATCTTAAACATTTGCATGTAAGCCTCTGGCTGATAGTTCTCCTTTCAAGATGTCAGCATCCTTTTCCGTGACATTTTCCTAGTTTTCTTTTTTTCGCATTGCTCGAAAGTTTTTGTGAGCATGGTTGCATCTACNTGATACTTTTCCTACATGCTTATATTCCGCAACGCCCACGCCAACTTGGAAAGCTAGAAAAAAAATCAAAATTCTTTGAGTACTAGAAATCTCGACATAATAGTAGAGACTTTNNGNGTCCATTCCAATTAAGTCNGGATGACTTTACAATGACTTTTNGAAAAGANAAANACAGTAANGNCCAAAAATCCTGGGACTTTTACCNCTTTGAGTTGTANTGCNCCCCGAANTTNGAATTTAGGCCCNGCNNAATGGAATAAAANCCAGCCNAAGGTNG : G S b m z %/<DPZeov
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$$%$2$=$H$T$`$k$w$$$$$$$$$$$% % %% %,%:%E%P%\%i%s%}%%%%%%%%%%%&&& &(&5&B&L&X&d&p&y&&&&&&&&&&&''''$'2'<'H'R'^'i'q'}'''''''''''((( ('(2(?(H(S(^(i(t(((((((((((())) )()3)?)G)Q)])j)r){)))))))))))** **%*.*9*D*N*Y*c*n*z************++++$+1+<+F+Q+\+g+q+|++++++++++++, ,, ,(,0,<,G,O,Z,d,o,{,,,,,,,,,,,,--- -)-4-@-K-T-]-g-s------------....".+.7.C.M.X.c.n.y............// // /)/3/=/G/Q/\/f/p/{///////////000 0'020=0H0S0^0h0r0|000000000000111 1(111=1G1Q1[1e1q1|11111111111112222%2/2<2I2R2[2dBasecaller-3730POP7SR.bcp AGNCCNGTCATTGACACAAACTCTATCCCATGCTCAGTATCGACGACGTCTCCGCACTGCAACTGCTGCACGAATTCAGAAGGGTTTGATTCGGTACCTCTACATTATCATTGATTTCTCCAGGGTATGTAAAATCCTATATGATTGCAAAATAACTGCAGTATTGTAATTGGTTACTGACATTTAAAGGTTTAATTTTATTTTTTTTTGCAAAATATGAAACACTTATTTAAGCATCTATAGTTATCTGTTTGTAAATGAGTTATCTCCGGAAGAAGTTAATCAGGTTCAATTTCCCAGACAATCTGCTGATCTTCTTCTGATGCTAGCAAATTGTTTCTTAAGAAGGAATACAACAGGGTCCTTTTTAAAGAACCAGCAGCTCTTCGTTTTATGGATTTCTATTTGCATCTAGGGCTGAAGATTTAGTGATTGCAACTGGTTGATTATGCTATGAGATATTGGTAAAACTAAGCAATTGGGTCGTCTTTGATCAGTTTAATGAATATTCAAGTGAATACCTACTTTCTGTTTTGATGTCACCATATTTCTTTAAACCACGTTTTGAAATTCTAAAATTGTGAATTGAGTTTATAAGACAATTATTGCATTTTGAAAATGTCCCTTAATGGGGTTGTGCTTATCACTGCATTGGACGTTTTCTTTTATCATCGACAAGGTGCTTTAACAGACAATCTTAAACATTTGCATGTAAGCCTCTGGCTGATAGTTCTCCTTTCAAGATGTCAGCATCCTTTTCCGTGACATTTTCCTAGTTTTCTTTTTTTCGCATTGCTCGAAAGTTTTTGTGAGCATGGTTGCATCTACNTGATACTTTTCCTACATGCTTATATTCCGCAACGCCCACGCCAACTTGGAAAGCTAGAAAAAAAATCAAAATTCTTTGAGTACTAGAAATCTCGACATAATAGTAGAGACTTTNNGNGTCCATTCCAATTAAGTCNGGATGACTTTACAATGACTTTTNGAAAAGANAAANACAGTAANGNCCAAAAATCCTGGGACTTTTACCNCTTTGAGTTGTANTGCNCCCCGAANTTNGAATTTAGGCCCNGCNNAATGGAATAAAANCCAGCCNAAGGTNG : G S b m z %/<DPZeov
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Po CTTL $ CpEP DATA ? J DATA ? J DATA ? J < DATA ? J ~ DATA DATA l DATA DATA DATA 2g d 5 DATA
2g d C DATA 2g d f DATA 2g d DSam DySN $ Dye# DyeN $ DyeN $ DyeN $ DyeN $ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
$l EVNT $x EVNT $ EVNT $ FTab 0
FVoc / FWO_ GATC Feat / / / GTyp ! ! $ InSc InVt LANE % LIMS ! ! $ LNTD $ LsrP a MCHN MODF % MODL 3730 NAVG NLNE 0 OFFS PBAS R R m PBAS R R ~ PCON R R z PCON R R PDMF Z# PDMF / PLOC R q PLOC R ? PXLB RGNm PY RMXV 1 RMdN Ht RMdV 2 RMdX H RPrN P- RPrV 2 RUND
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RUNT 6# RUNT ' RUNT
RUNT ' Rate $ RevC RunN # # P6 S/N% / SCAN ? SMED
SMLt SMPL Z SPAC AW SPAC z SPAC AW SVER 2.0 SVER c SVER , , $@ ScSt Scal @ Scan ? TUBE D10 Tmpr < User Tom <8s*5c'iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@rR&4_L/Fa$3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`FJ`_saiY2]@R ,$gEn-8hP3W4KRJxG57'9\47.<<_/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jblf~&]==6XXI p*][oTsr*G6`VT/}}1%j-]Tsz^e9Su3]w ASPF ASPt ASPt AUDT \ \ <] B1Pt B1Pt BufT P| CTID Ph CTNM
Po CTTL $ CpEP DATA ? J DATA ? J DATA ? J < DATA ? J ~ DATA DATA l DATA DATA DATA 2g d 5 DATA
2g d C DATA 2g d f DATA 2g d DSam DySN $ Dye# DyeN $ DyeN $ DyeN $ DyeN $ DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
$l EVNT $x EVNT $ EVNT $ FTab 0
FVoc / FWO_ GATC Feat / / / GTyp ! ! $ InSc InVt LANE % LIMS ! ! $ LNTD $ LsrP a MCHN MODF % MODL 3730 NAVG NLNE 0 OFFS PBAS R R m PBAS R R ~ PCON R R z PCON R R PDMF Z# PDMF / PLOC R q PLOC R ? PXLB RGNm PY RMXV 1 RMdN Ht RMdV 2 RMdX H RPrN P- RPrV 2 RUND
RUND
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RUNT 6# RUNT ' RUNT
RUNT ' Rate $ RevC RunN # # P6 S/N% / SCAN ? SMED
SMLt SMPL Z SPAC AW SPAC z SPAC AW SVER 2.0 SVER c SVER , , $@ ScSt Scal @ Scan ? TUBE D10 Tmpr < User Tom