Clone hba-250i21

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Clone
Clone name
hba-250i21
LE_HBa0250I21
C06HBa0250I21
P250I21
Clone type
BAC
Contamination
none
Estimated length
48,389
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
MiTomato-EXPEN 2000plate 13G2
Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
66-13 1CompositeFluorescencePhase58.5%
66-14 1CompositeFluorescencePhase60.3%
66-19 1CompositeFluorescencePhase64.4%
66-22 1CompositeFluorescencePhase62.5%
66-28 1CompositeFluorescencePhase68.4%
66-31 1CompositeFluorescence_copyPhase64.1%
66-9 1CompositeFluorescencePhase67.9%
Mean±sd63.7%±3.7%

Given an estimated length of 3.7µm for chromosome 6P and the mean percentage above, this BAC is approximately 2.4µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 6 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 6
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
148,257 bp
C06HBa0250I21.2
AC209512.1 (GenBank)
[View]
[Download fasta]
[Download full submission] (0.14 MB)
End Sequences
None
Warnings
  • poor correspondence with in vitro restriction fragments
    (match score below 0.7)
  • sequence length 148,257 is 206.4% different from estimated length 48,389
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (4)
9670, 10760, 12215, 15744
HindIII in silico (36)
1071, 1123, 1215, 1313, 1342, 1755, 1826, 1884, 1893, 2022, 2137, 2320, 2335, 2469, 2499, 2593, 2708, 2864, 3236, 3256, 3322, 3564, 3759, 3801, 3962, 4008, 4053, 4511, 4992, 5070, 5304, 5645, 9567, 10371, 12000, 15444
Fragment Lengths Match Score
0.24
Annotations  
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LE_HBa0250I21 has no browsable sequence annotations
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