Clone hba-27b5

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Clone
Clone name
hba-27b5
LE_HBa0027B05
C11HBa0027B05
P027B05
Clone type
BAC
Contamination
none
Estimated length
150,122
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
Bs4Tomato-EXPEN 2000plate 13B1
Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
290-02 1CompositeFluorescencePhase61.1%
282-02 1CompositeFluorescencePhase68.7%
282-03 1CompositeFluorescencePhase60.4%
290-10 1CompositeFluorescencePhase62.3%
282-11 1CompositeFluorescencePhase64.7%
282-12a 1CompositeFluorescencePhase68.1%
282-12b 1CompositeFluorescencePhase62.0%
282-13 1CompositeFluorescencePhase63.1%
282-15 1CompositeFluorescencePhase61.8%
282-18 1CompositeFluorescencePhase60.2%
282-19 1CompositeFluorescencePhase63.6%
290-21 1CompositeFluorescencePhase65.6%
290-22 1CompositeFluorescencePhase61.6%
290-23 1CompositeFluorescencePhase63.6%
290-34 1CompositeFluorescencePhase60.6%
290-36 1CompositeFluorescencePhase64.3%
290-38 1CompositeFluorescencePhase66.9%
290-40 1CompositeFluorescencePhase65.9%
Mean±sd63.6%±2.6%

Given an estimated length of 6.5µm for chromosome 11P and the mean percentage above, this BAC is approximately 4.1µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 11 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 11
HTGS phase
3 - finished
Sequencing Status
in_progress
Sequencing Organization
Indian Initiative on Tomato Genome Sequencing
Full Sequence
169,464 bp
C11HBa0027B05.2
AC212314.2 (GenBank)
[View]
[Download fasta]
[Download full submission] (836.96 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (32)
1123, 1277, 1341, 1415, 1516, 1620, 1906, 1970, 2027, 2416, 2416, 2924, 2995, 3342, 3397, 3476, 3787, 3914, 3914, 4022, 4451, 4590, 4766, 5654, 5654, 5837, 5924, 6353, 10912, 11658, 16172, 17353
HindIII in silico (40)
1110, 1120, 1175, 1220, 1253, 1264, 1301, 1334, 1337, 1385, 1413, 1434, 1521, 1614, 1891, 1947, 2412, 2428, 2450, 2923, 3015, 3355, 3423, 3500, 3854, 3948, 3972, 4062, 4448, 4557, 4661, 5314, 5394, 5547, 5653, 6194, 10842, 11529, 15793, 16895
Fragment Lengths Match Score
0.89
Annotations  
Browse
LE_HBa0027B05 has no browsable sequence annotations
Download
temporarily unavailable
User comments [Add comment] 
Posted by Robert Buels (curator) on 2007-10-16 14:14:58 Delete  
sequenced by chromosome 5 project, but subsequently localized by Stack lab FISH to chromosome 11
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