Clone hba-316e10

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Clone
Clone name
hba-316e10
LE_HBa0316E10
C00HBa0316E10
P316E10
Clone type
BAC
Contamination
none
Estimated length
115,174
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg157 : [coherent], 9 members:
LE_HBa0018K15, LE_HBa0068A12, LE_HBa0145G05, LE_HBa0147G09, LE_HBa0165C11, C04HBa0171B21, LE_HBa0297M18, LE_HBa0308D07, C00HBa0316E10
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
270-07 1CompositeFluorescencePhase57.9%
295-09 1CompositeFluorescencePhase55.1%
270-11 1CompositeFluorescence57.6% 
270-13 1CompositeFluorescencePhase61.4%
295-20 1CompositeFluorescencePhase58.7%
295-21 1CompositeFluorescencePhase56.5%
270-21 1CompositeFluorescencePhase61.0%
295-24 1CompositeFluorescencePhase55.4%
270-28 1CompositeFluorescencePhase61.3%
270-29 1CompositeFluorescencePhase57.6%
270-30 1CompositeFluorescencePhase61.5%
270-31 1CompositeFluorescencePhase61.3%
295-35 1CompositeFluorescencePhase58.4%
295-36 1CompositeFluorescencePhase59.5%
295-38 1CompositeFluorescencePhase58.5%
295-40 1CompositeFluorescencePhase56.0%
295-41 1CompositeFluorescencePhase53.6%
Mean±sd58.3%±2.5%

Given an estimated length of 8.0µm for chromosome 11Q and the mean percentage above, this BAC is approximately 4.7µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL Mapping 
Assigned to project
Italy
IL-mapped to chromosome
11
IL-mapped to IL segment
-
IL mapping notes
-
Sequencing  
This clone is registered to be sequenced, but has not been successfully mapped to any chromosome.

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Sequencing Project
Chromosome unmapped
HTGS phase
none
Sequencing Status
in_progress
Sequencing Organization
not recorded
Full Sequence
Sequence not available.
End Sequences
None
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (24)
1271, 2086, 2155, 2307, 2355, 2422, 2467, 2917, 3165, 3664, 3706, 3919, 4404, 4527, 4527, 4830, 5249, 6126, 6399, 6727, 6727, 8671, 10738, 13815
Annotations  
Browse
LE_HBa0316E10 has no browsable sequence annotations
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