Clone hba-99f14

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Clone
Clone name
hba-99f14
LE_HBa0099F14
C09HBa0099F14
P099F14
Clone type
BAC
Contamination
none
Estimated length
102,884
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg160 : [coherent], 2 members:
C09HBa0099F14, LE_HBa0296O18
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
351-04 1CompositeFluorescencePhase61.4%
351-06 1CompositeFluorescencePhase62.7%
351-07 1CompositeFluorescencePhase62.6%
351-09 1CompositeFluorescencePhase62.2%
351-11 1CompositeFluorescencePhase61.9%
351-12 1CompositeFluorescencePhase61.3%
351-16 1CompositeFluorescencePhase58.0%
351-31 1CompositeFluorescencePhase62.2%
351-34 1CompositeFluorescencePhase61.6%
351-35 1CompositeFluorescencePhase64.1%
351-39 1CompositeFluorescencePhase61.9%
351-41 1CompositeFluorescencePhase57.8%
351-42 1CompositeFluorescencePhase61.3%
351-43 1CompositeFluorescencePhase62.6%
351-44 1CompositeFluorescencePhase62.2%
351-45 1CompositeFluorescencePhase61.1%
351-46 1CompositeFluorescencePhase64.3%
351-48 1CompositeFluorescencePhase58.9%
351-49a 1CompositeFluorescencePhase59.5%
351-49b 1CompositeFluorescencePhase57.7%
351-50 1CompositeFluorescencePhase60.6%
Mean±sd61.2%±1.9%

Given an estimated length of 9.7µm for chromosome 9Q and the mean percentage above, this BAC is approximately 5.9µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 9 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 9
HTGS phase
2 - fragmented, ordered
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
107,857 bp
C09HBa0099F14.1
EU139069.1 (GenBank)
[View]
[Download fasta]
[Download full submission] (484.88 MB)
End Sequences
Warnings
  • sequence is not finished to HTGS3
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (21)
1708, 1708, 1761, 2448, 2465, 2980, 3200, 3516, 3579, 3710, 3850, 4483, 5207, 5526, 5804, 6289, 6331, 7352, 8098, 10584, 12285
HindIII in silico (20)
1063, 1318, 1693, 1765, 2478, 2993, 3202, 3545, 3608, 3750, 3876, 4891, 4990, 5194, 5443, 7358, 8013, 10227, 12517, 13786
Fragment Lengths Match Score
0.94
Annotations  
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LE_HBa0099F14 has no browsable sequence annotations
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