Clone hba-208m24

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Clone
Clone name
hba-208m24
LE_HBa0208M24
C01HBa0208M24
P208M24
Clone type
BAC
Contamination
none
Estimated length
126,129
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg985 : [coherent], 8 members:
LE_HBa0016F06, LE_HBa0120H17, LE_HBa0193B10, LE_HBa0198K04, C01HBa0208M24, LE_HBa0234E03, LE_HBa0245I04, LE_HBa0266F23
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manual 
Marker Publication
unpublished
Submitted by
Lukas Mueller (Boyce Thompson Institute for Plant Research)
Comment
Manual associations of BACs with mapped BAC ends. Mapping by Yimin Xu, Cornell University
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
604-04 1CompositeFluorescencePhase52.5%
604-08 1CompositeFluorescencePhase55.1%
604-12 1CompositeFluorescencePhase55.8%
604-16 1CompositeFluorescencePhase53.0%
604-25 1CompositeFluorescencePhase55.8%
604-28 1CompositeFluorescencePhase54.4%
604-39 1CompositeFluorescencePhase55.1%
604-40 1CompositeFluorescencePhase55.0%
604-47 1CompositeFluorescencePhase57.3%
604-62 1CompositeFluorescencePhase51.3%
604-74 1CompositeFluorescencePhase51.7%
604-88 1CompositeFluorescencePhase53.0%
Mean±sd54.2%±1.8%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 10.9µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
3 - finished
Sequencing Status
in_progress
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
154,446 bp
C01HBa0208M24.1
AC239433.3 (GenBank)
[View]
[Download fasta]
[Download full submission] (0.10 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (25)
1659, 1783, 1791, 1870, 2644, 3004, 3129, 3584, 3611, 3884, 3914, 4325, 4671, 5383, 5759, 6180, 6271, 6271, 6630, 7227, 7476, 8190, 8720, 8720, 9433
HindIII in silico (40)
1020, 1021, 1122, 1246, 1432, 1515, 1646, 1661, 1681, 1776, 1795, 1801, 1848, 1860, 1949, 1962, 2265, 2471, 2689, 3005, 3174, 3600, 3669, 3681, 3929, 3992, 4336, 4552, 5093, 5463, 5938, 6047, 6158, 6575, 7168, 7429, 8098, 8609, 8638, 9241
Fragment Lengths Match Score
0.78
Annotations  
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LE_HBa0208M24 has no browsable sequence annotations
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