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### Clone hba-6e18

 Clone and library
Clone
Clone name
hba-6e18
LE_HBa0006E18
C04HBa0006E18
P006E18
Clone type
BAC
Contamination
none
Estimated length
125,909
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
 Physical mapping
 Fingerprint Contig Builds (FPC)
Tomato FPC (AGI 2005)
ctg1173 : [coherent], 10 members:
C04HBa0006E18, LE_HBa0164F08, LE_HBa0165L02, LE_HBa0171J15, LE_HBa0176B07, LE_HBa0190G18, LE_HBa0218E22, LE_HBa0256N02, LE_HBa0273D07, LE_HBa0336L13
 Marker matches - overgo what's this?
Conflicted matches
None

 Marker matches - BLAST None
 Marker matches - manual None
 Physical map (from marker matches)
 FISH map
 FISH images
(percentage of arm length)
175-03a 1CompositeFluorescencePhase22.2%
175-03b 1CompositeFluorescencePhase26.2%
175-03c 1CompositeFluorescencePhase23.4%
175-04 1CompositeFluorescencePhase22.8%
175-06 1CompositeFluorescencePhase26.6%
175-08 1CompositeFluorescencePhase27.8%
175-11 1CompositeFluorescencePhase26.0%
175-17 1CompositeFluorescencePhase24.2%
175-19 1CompositeFluorescencePhase25.5%
175-20 1CompositeFluorescencePhase23.2%
175-21 1CompositeFluorescencePhase22.6%
175-25 1CompositeFluorescencePhase23.3%
Mean±sd24.5%±1.8%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 3.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
 IL Mapping None
 Sequencing
This clone is being sequenced by the Chromosome 4 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 4
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
End Sequences
Warnings
none
FTP
Chromosome Assembly
Restriction Fragments
 HindIII in vitro (32) 1025, 1205, 1205, 1313, 1450, 1515, 1586, 1665, 1665, 2263, 2318, 2441, 2607, 2972, 3069, 3294, 3333, 3441, 3598, 3770, 3770, 3770, 4076, 4310, 6476, 6476, 6836, 7648, 8490, 8490, 9299, 10533 HindIII in silico (30) 1026, 1193, 1204, 1309, 1443, 1513, 1578, 1645, 1671, 2277, 2350, 2473, 2621, 2951, 3063, 3282, 3340, 3463, 3646, 3801, 3803, 4085, 4301, 6354, 6446, 6842, 8289, 8437, 9217, 10213 Fragment Lengths Match Score 0.96
 Annotations
Browse
LE_HBa0006E18 has no browsable sequence annotations