Clone hba-251j13

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Clone
Clone name
hba-251j13
LE_HBa0251J13
C05HBa0251J13
P251J13
Clone type
BAC
Contamination
none
Estimated length
98,949
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg4132 : [coherent], 1 members:
C05HBa0251J13
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
221-08 1CompositeFluorescencePhase97.1%
222-10 1CompositeFluorescencePhase95.5%
221-12 1CompositeFluorescencePhase98.7%
222-12 1CompositeFluorescencePhase100.0%
222-13 1CompositeFluorescencePhase96.8%
222-16 1CompositeFluorescencePhase98.7%
221-16 1CompositeFluorescencePhase100.0%
221-17 1CompositeFluorescencePhase96.9%
222-17 1CompositeFluorescencePhase96.9%
221-18 1CompositeFluorescencePhase100.0%
222-20 1CompositeFluorescencePhase97.6%
221-21 1CompositeFluorescencePhase97.9%
221-24 1CompositeFluorescencePhase100.0%
221-25 1CompositeFluorescencePhase97.5%
221-26 1CompositeFluorescencePhase96.9%
Mean±sd98.0%±1.4%

Given an estimated length of 7.7µm for chromosome 5Q and the mean percentage above, this BAC is approximately 7.5µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 5 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 5
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Indian Agricultural Research Institute
Full Sequence
99,174 bp
C05HBa0251J13.2
AC186292.3 (GenBank)
[View]
[Download fasta]
[Download full submission] (657.08 MB)
End Sequences
None
Warnings
none
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (31)
1205, 1239, 1315, 1315, 1417, 1494, 1523, 1588, 1773, 1871, 2010, 2010, 2185, 2251, 2293, 2474, 2839, 3202, 3346, 3420, 3657, 3657, 3956, 4558, 5192, 5427, 5968, 5968, 6168, 6562, 7066
HindIII in silico (29)
1052, 1087, 1186, 1225, 1293, 1297, 1412, 1492, 1592, 1775, 1853, 1975, 2198, 2257, 2499, 2843, 3207, 3348, 3435, 3673, 3694, 3970, 4510, 5706, 5764, 5936, 6543, 7092, 8474
Fragment Lengths Match Score
0.95
Annotations  
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LE_HBa0251J13 has no browsable sequence annotations
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