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Clone hba-305j13

Clone and library  
Clone
Clone name
hba-305j13
LE_HBa0305J13
C01HBa0305J13
P305J13
Clone type
BAC
Contamination
none
Estimated length
128,984
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg680 : [coherent], 5 members:
LE_HBa0105E12, LE_HBa0198N17, LE_HBa0236O08, LE_HBa0244A10, C01HBa0305J13
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
C2_At5g35360Tomato-EXPEN 2000plate 13D2
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 1
FISH map 
View on FISH map chromosome 1
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
457-03 1CompositeFluorescencePhase75.3%
457-10 1CompositeFluorescencePhase79.4%
457-11 1CompositeFluorescencePhase81.5%
457-15 1CompositeFluorescencePhase76.3%
457-22 1CompositeFluorescencePhase79.2%
457-24 1CompositeFluorescencePhase78.0%
457-28 1CompositeFluorescencePhase77.7%
457-38 1CompositeFluorescencePhase77.9%
457-40a 1CompositeFluorescencePhase79.6%
457-40b 1CompositeFluorescencePhase80.4%
457-43 1CompositeFluorescencePhase78.3%
457-44 1CompositeFluorescencePhase76.0%
Mean±sd78.3%±1.8%

Given an estimated length of 6.7µm for chromosome 1P and the mean percentage above, this BAC is approximately 5.2µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
143,279 bp
C01HBa0305J13.1
AC239435.2 (GenBank) 		[View]
[Download fasta]
[Download full submission] (0.09 MB)
End Sequences
None
Warnings
none
FTP
Chromosome 1 Sequencing repository (FTP)
Chromosome Assembly
View on AGP map chromosome 1
  
Restriction Fragments
HindIII in vitro (28)
1547, 1657, 1713, 1843, 1984, 2016, 2104, 2239, 2378, 2540, 2860, 3163, 3367, 3588, 3836, 4149, 4149, 4852, 4852, 5503, 6067, 6277, 6934, 8469, 9055, 10284, 10284, 11274
HindIII in silico (35)
1008, 1010, 1184, 1264, 1323, 1348, 1501, 1569, 1647, 1660, 1704, 1965, 2002, 2102, 2256, 2410, 2566, 2866, 3157, 3402, 3626, 3902, 4169, 4223, 4708, 4727, 5170, 5815, 6139, 6937, 8359, 9062, 10226, 10231, 11123
Fragment Lengths Match Score
0.87
Annotations  
Browse
LE_HBa0305J13 has no browsable sequence annotations
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temporarily unavailable
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 and  bp
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End Sequences

e.g. crystallin
Overgo match to marker what's this?
Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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