Clone hba-309l13

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Clone
Clone name
hba-309l13
LE_HBa0309L13
C05HBa0309L13
P309L13
Clone type
BAC
Contamination
none
Estimated length
134,546
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg1727 : [coherent], 8 members:
LE_HBa0041C18, LE_HBa0055G23, LE_HBa0237E15, C05HBa0244P17, LE_HBa0255M10, LE_HBa0271P05, LE_HBa0294K10, C05HBa0309L13
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
C2_At2g01110Tomato-EXPEN 2000plate 13C2
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 5
FISH map 
View on FISH map chromosome 5
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
286-02 1CompositeFluorescencePhase74.0%
286-03 1CompositeFluorescencePhase75.9%
286-04 1CompositeFluorescencePhase76.8%
286-07 1CompositeFluorescencePhase79.2%
286-10 1CompositeFluorescencePhase76.9%
286-13 1CompositeFluorescencePhase77.2%
286-14 1CompositeFluorescencePhase75.6%
286-15 1CompositeFluorescencePhase73.9%
286-18 1CompositeFluorescencePhase79.3%
286-20 1CompositeFluorescencePhase78.1%
286-26 1CompositeFluorescencePhase76.8%
286-28 1CompositeFluorescencePhase75.4%
286-32 1CompositeFluorescencePhase76.9%
286-33 1CompositeFluorescencePhase75.5%
286-34 1CompositeFluorescencePhase76.3%
286-35 1CompositeFluorescencePhase74.6%
286-36 1CompositeFluorescencePhase79.8%
Mean±sd76.6%±1.8%

Given an estimated length of 7.4µm for chromosome 5P and the mean percentage above, this BAC is approximately 5.7µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 5 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 5
HTGS phase
2 - fragmented, ordered
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
126,922 bp
C05HBa0309L13.1
AC212304.1 (GenBank) 		[View]
End Sequences
None
Warnings
  • sequence is not finished to HTGS3
 FTP
Chromosome 5 Sequencing repository (FTP)
Chromosome Assembly
View on AGP map chromosome 5
  
Restriction Fragments
HindIII in vitro (30)
1181, 1294, 1405, 1529, 1551, 1744, 1882, 1914, 2050, 2050, 2402, 2402, 2579, 2757, 2955, 3056, 3310, 3353, 4336, 4688, 6240, 6738, 7172, 7254, 7382, 8366, 8466, 10018, 11368, 13104
HindIII in silico (32)
1160, 1165, 1276, 1402, 1482, 1528, 1561, 1725, 1856, 1903, 2021, 2023, 2043, 2051, 2419, 2447, 2577, 2772, 2943, 3048, 3345, 3399, 4332, 4598, 6047, 6719, 7213, 8347, 8393, 8807, 9883, 12878
Fragment Lengths Match Score
0.96
Annotations  
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LE_HBa0309L13 has no browsable sequence annotations
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End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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