Clone hba-323c4

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Clone
Clone name
hba-323c4
LE_HBa0323C04
C00HBa0323C04
P323C04
Clone type
BAC
Contamination
none
Estimated length
141,188
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
843-03 1CompositeFluorescencePhase45.9%
843-15 1CompositeFluorescencePhase50.0%
843-24 1CompositeFluorescencePhase47.8%
843-25 1CompositeFluorescencePhase49.0%
843-26 1CompositeFluorescencePhase49.5%
843-31 1CompositeFluorescencePhase51.7%
843-32 1CompositeFluorescencePhase51.3%
843-33 1CompositeFluorescencePhase47.3%
Mean±sd49.1%±2.0%

Given an estimated length of 5.1µm for chromosome 4P and the mean percentage above, this BAC is approximately 2.5µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL Mapping 
Assigned to project
Korea
IL-mapped to chromosome
-
IL-mapped to IL segment
-
IL mapping notes
-
Sequencing  
This clone is registered to be sequenced, but has not been successfully mapped to any chromosome.

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Sequencing Project
Chromosome unmapped
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Korea Research Institute of Bioscience and Biotechnology
Full Sequence
162,185 bp
C00HBa0323C04.2
AC215440.2 (GenBank)
[View]
[Download fasta]
[Download full submission] (335.81 MB)
End Sequences
None
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (29)
1612, 1702, 1771, 1868, 1887, 2082, 2173, 2173, 2255, 2328, 2328, 2437, 2880, 3801, 3801, 4106, 4215, 4749, 5872, 5872, 6089, 6130, 6646, 6847, 8210, 9514, 10626, 11005, 16209
HindIII in silico (41)
1049, 1052, 1074, 1107, 1155, 1159, 1168, 1203, 1322, 1385, 1397, 1624, 1698, 1764, 1833, 1861, 1888, 2077, 2167, 2180, 2266, 2339, 2342, 2435, 2889, 3818, 3823, 4101, 4203, 4629, 5577, 5611, 5844, 5914, 6635, 6864, 8105, 9505, 10664, 10932, 15952
Fragment Lengths Match Score
0.81
Annotations  
Browse
LE_HBa0323C04 has no browsable sequence annotations
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