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Clone hba-45n22

Clone and library  
Clone
Clone name
hba-45n22
LE_HBa0045N22
C12HBa0045N22
P045N22
Clone type
BAC
Contamination
none
Estimated length
91,519
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this?None
Marker matches - BLAST 
MarkerEvalueIdentity %ScoreParams
T06595e-10160.00evalue cutoff: 1e-10; min len = 25; min identity=0.96
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
9-12 1CompositeFluorescencePhase100.0%
9-15 1CompositeFluorescencePhase95.0%
9-17 1CompositeFluorescencePhase96.6%
9-18 1CompositeFluorescencePhase96.5%
9-1a 1CompositeFluorescencePhase95.6%
9-5 1CompositeFluorescencePhase93.4%
9-7 1CompositeFluorescencePhase97.6%
9-8 1CompositeFluorescencePhase100.0%
Mean±sd96.8%±2.3%

Given an estimated length of 6.8µm for chromosome 12P and the mean percentage above, this BAC is approximately 6.6µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 12 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 12
HTGS phase
none
Sequencing Status
none
Sequencing Organization
not recorded
Full Sequence
Sequence not available.
End Sequences
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (22)
1404, 1500, 1740, 1966, 2128, 2158, 2196, 2736, 2746, 2863, 3225, 3339, 3637, 4933, 5461, 5905, 6087, 6323, 6543, 6621, 8890, 9118
Annotations  
Browse
LE_HBa0045N22 has no browsable sequence annotations
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