### Clone hba-31m5

 Clone and library
Clone
Clone name
hba-31m5
LE_HBa0031M05
C03HBa0031M05
P031M05
Clone type
BAC
Contamination
none
Estimated length
151,440
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
 Physical mapping
 Fingerprint Contig Builds (FPC)
Tomato FPC (AGI 2005)
ctg1199 : [coherent], 11 members:
C03HBa0031M05, C03HBa0034O02, C03HBa0070P03, C03HBa0223J13, C03HBa0239J12, C03HBa0257C07, C03HBa0277G02, C03HBa0288D21, C03HBa0322O07, C03HBa0174D19, C03HBa0190L06
 Marker matches - overgo what's this?
Conflicted matches
None

 Marker matches - BLAST None
 Marker matches - manual None
 Physical map (from marker matches)
 FISH map
 FISH images
(percentage of arm length)
342-04 1CompositeFluorescencePhase61.5%
342-06 1CompositeFluorescencePhase62.6%
342-07 1CompositeFluorescencePhase58.2%
342-08 1CompositeFluorescencePhase58.9%
342-09 1CompositeFlourescencePhase60.1%
342-19 1CompositeFluorescencePhase64.3%
342-20 1CompositeFluorescencePhase60.2%
342-23 1CompositeFluorescencePhase66.8%
342-24 1CompositeFluorescencePhase62.1%
342-28 1CompositeFluorescencePhase62.5%
342-34 1CompositeFluorescencePhase64.5%
342-35 1CompositeFluorescencePhase63.7%
342-44 1CompositeFluorescencePhase63.8%
342-46 1CompositeFluorescencePhase61.8%
342-48 1CompositeFluorescencePhase57.5%
342-49 1CompositeFluorescencePhase63.1%
342-50 1CompositeFluorescencePhase63.8%
Mean±sd62.1%±2.5%

Given an estimated length of 4.8µm for chromosome 3P and the mean percentage above, this BAC is approximately 3.0µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
 IL Mapping None
 Sequencing
This clone is being sequenced by the Chromosome 3 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 3
HTGS phase
2 - fragmented, ordered
Sequencing Status
complete
Sequencing Organization
Full Sequence
End Sequences
Warnings
• sequence is not finished to HTGS3
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly

Restriction Fragments
 HindIII in vitro (33) 1504, 1565, 1633, 1785, 2034, 2216, 2338, 2478, 2478, 2541, 2804, 2804, 2916, 2916, 2982, 3107, 3274, 3324, 3593, 3973, 4478, 4636, 5446, 5446, 5866, 6022, 7183, 7521, 8039, 8039, 11236, 12070, 13193 HindIII in silico (41) 1086, 1095, 1102, 1126, 1229, 1497, 1514, 1573, 1639, 1775, 2013, 2216, 2352, 2478, 2508, 2556, 2801, 2812, 2839, 2914, 2932, 2995, 3115, 3280, 3309, 3351, 3623, 4010, 4261, 4451, 4564, 5134, 5180, 5554, 5751, 7142, 7214, 7432, 11143, 11893, 12899 Fragment Lengths Match Score 0.88
 Annotations
Browse
LE_HBa0031M05 has no browsable sequence annotations