### Clone hba-69e17

 Clone and library
Clone
Clone name
hba-69e17
LE_HBa0069E17
C01HBa0069E17
P069E17
Clone type
BAC
Contamination
none
Estimated length
136,801
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
 Physical mapping
 Fingerprint Contig Builds (FPC)
not present in Tomato FPC (AGI 2005)
 Marker matches - overgo what's this?
Plausible matches
ProbeMapPlateWell
CT87Tomato-EXPEN 2000plate 3C8
Conflicted matches
None

 Marker matches - BLAST None
 Marker matches - manual None
 Physical map (from marker matches)
 FISH map
 FISH images
(percentage of arm length)
187-15 1CompositeFluorescencePhase83.2%
187-16 1CompositeFluorescencePhase83.0%
187-21 1CompositeFluorescencePhase82.9%
187-24 1CompositeFluorescencePhase85.1%
187-27 1CompositeFluorescencePhase84.4%
187-28 1CompositeFluorescencePhase84.2%
187-29 1CompositeFluorescencePhase82.8%
187-30 1CompositeFluorescencePhase83.6%
187-35 1CompositeFluorescencePhase84.1%
187-40 1CompositeFluorescencePhase85.0%
187-41 1CompositeFluorescencePhase83.6%
187-42 1CompositeFluorescencePhase85.5%
187-47 1CompositeFluorescencePhase84.2%
187-51 1CompositeFluorescencePhase85.9%
Mean±sd84.1%±1.0%

Given an estimated length of 6.7µm for chromosome 1P and the mean percentage above, this BAC is approximately 5.6µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
 IL Mapping None
 Sequencing
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
1 - fragmented, unordered
Sequencing Status
complete
Sequencing Organization
Agencourt
Full Sequence
End Sequences
Warnings
• sequence is not finished to HTGS3
FTP
Chromosome Assembly
Restriction Fragments
 HindIII in vitro (28) 1767, 1767, 1813, 1866, 1886, 1964, 2027, 2152, 2204, 2285, 2681, 2842, 2842, 3346, 3645, 3645, 3764, 3848, 4225, 5340, 5675, 6237, 6685, 8929, 9050, 9643, 9818, 24855 HindIII in silico (35) 1089, 1120, 1350, 1404, 1446, 1510, 1568, 1636, 1743, 1768, 1806, 1861, 1882, 1952, 2018, 2154, 2225, 2310, 2707, 2846, 3373, 3653, 3694, 3798, 3879, 4254, 5059, 5373, 6059, 6638, 8800, 9096, 9541, 9704, 24878 Fragment Lengths Match Score 0.87
 Annotations
Browse
LE_HBa0069E17 has no browsable sequence annotations