Clone hba-55e23

Clone and library  
Clone
Clone name
hba-55e23
LE_HBa0055E23
C01HBa0055E23
P055E23
Clone type
BAC
Contamination
none
Estimated length
96,132
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg4104 : [coherent], 3 members:
LE_HBa0001J06, C01HBa0055E23, LE_HBa0066A04
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLAST 
MarkerEvalueIdentity %ScoreParams
cLPT-5-K211e-1070.985383.00evalue cutoff: 1e-10; min len = 25; min identity=0.96
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
692-01 1CompositeFluorescencePhase35.5%
692-09 1CompositeFluorescencePhase35.1%
692-13 1CompositeFluorescencePhase35.5%
692-14 1CompositeFluorescencePhase36.7%
692-20 1CompositeFluorescencePhase36.3%
692-21 1CompositeFluorescencePhase35.3%
692-27 1CompositeFluorescencePhase36.7%
692-28 1CompositeFluorescencePhase33.0%
692-33 1CompositeFluorescencePhase35.2%
692-34 1CompositeFluorescencePhase35.0%
692-36 1CompositeFluorescencePhase34.0%
692-37 1CompositeFluorescencePhase34.6%
692-46 1CompositeFluorescencePhase31.8%
692-50 1CompositeFluorescencePhase32.6%
692-51 1CompositeFluorescencePhase33.6%
692-58 1CompositeFluorescencePhase36.6%
Mean±sd34.8%±1.5%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 7.0µm±0.3µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL Mapping 
Assigned to project
USA
IL-mapped to chromosome
-
IL-mapped to IL segment
-
IL mapping notes
-
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
3 - finished
Sequencing Status
in_progress
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
96,711 bp
C01HBa0055E23.1
AC239438.2 (GenBank)
[View]
[Download fasta]
[Download full submission] (0.06 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (11)
1218, 1636, 3280, 5163, 6090, 7040, 8653, 9646, 14104, 14874, 24428
HindIII in silico (11)
1212, 1637, 3317, 4952, 5852, 7030, 8575, 9588, 13830, 14594, 24239
Fragment Lengths Match Score
0.99
Annotations  
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LE_HBa0055E23 has no browsable sequence annotations
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