Clone hba-140o23

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Clone
Clone name
hba-140o23
LE_HBa0140O23
C01HBa0140O23
P140O23
Clone type
BAC
Contamination
none
Estimated length
83,773
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2236 : [coherent], 2 members:
C01HBa0140O23, LE_HBa0246F17
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
657-06 1CompositeFluorescencePhase59.0%
657-11 1CompositeFluorescencePhase58.9%
657-16 1CompositeFluorescencePhase57.6%
657-19 1CompositeFluorescencePhase59.4%
657-24 1CompositeFluorescencePhase61.2%
657-28 1CompositeFluorescencePhase55.8%
657-29 1CompositeFluorescencePhase62.0%
657-32 1CompositeFluorescencePhase59.8%
657-36 1CompositeFluorescencePhase58.2%
657-38 1CompositeFluorescencePhase59.8%
657-40 1CompositeFluorescencePhase60.0%
657-43 1CompositeFluorescencePhase57.1%
657-49 1CompositeFluorescencePhase58.9%
657-50 1CompositeFluorescencePhase56.6%
657-56 1CompositeFluorescencePhase58.9%
657-58 1CompositeFluorescencePhase56.8%
657-60 1CompositeFluorescencePhase56.1%
Mean±sd58.6%±1.7%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 11.8µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
126,308 bp
C01HBa0140O23.1
AC238911.3 (GenBank)
[View]
[Download fasta]
[Download full submission] (0.08 MB)
End Sequences
Warnings
  • sequence length 126,308 is 50.8% different from estimated length 83,773
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (21)
1548, 1873, 2002, 2002, 2126, 2126, 2426, 2593, 2901, 2964, 3124, 3237, 3354, 3822, 3956, 4218, 5130, 6065, 6896, 7513, 13897
HindIII in silico (29)
1025, 1068, 1097, 1383, 1425, 1539, 1881, 1974, 2009, 2106, 2128, 2445, 2603, 2877, 2930, 3132, 3226, 3364, 3869, 4000, 4221, 4877, 5785, 6901, 7279, 7539, 7557, 13424, 13627
Fragment Lengths Match Score
0.83
Annotations  
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LE_HBa0140O23 has no browsable sequence annotations
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