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Clone hba-159c14

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Clone
Clone name
hba-159c14
LE_HBa0159C14
C01HBa0159C14
P159C14
Clone type
BAC
Contamination
none
Estimated length
117,805
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg4239 : [coherent], 3 members:
LE_HBa0018A19, C01HBa0057C07, C01HBa0159C14
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
453-01 1CompositeFluorescencePhase64.1%
453-04 1CompositeFluorescencePhase63.1%
658-11 1CompositeFluorescencePhase64.2%
658-19 1CompositeFluorescencePhase68.5%
658-20 1CompositeFluorescencePhase66.9%
658-21 1CompositeFluorescencePhase70.2%
658-23 1CompositeFluorescencePhase67.8%
658-24 1CompositeFluorescencePhase65.3%
453-25 1CompositeFluorescencePhase65.6%
658-27 1CompositeFluorescencePhase66.8%
453-32 1CompositeFluorescencePhase63.4%
453-33 1CompositeFluorescencePhase62.6%
453-34 1CompositeFluorscencePhase62.7%
453-35 1CompositeFluorescencePhase64.0%
453-36 1CompositeFluorescencePhase62.8%
453-38 1CompositeFluorescencePhase63.5%
453-41 1CompositeFluorescencePhase62.7%
658-43 1CompositeFluorescencePhase64.4%
453-44a 1CompositeFluorescencePhase63.5%
453-44b 1CompositeFluorescencePhase61.6%
453-45 1CompositeFluorescencePhase66.5%
453-48 1CompositeFluorescencePhase63.6%
658-49 1CompositeFluorescencePhase67.0%
Mean±sd64.8%±2.2%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 13.0µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Agencourt
Full Sequence
123,679 bp
C01HBa0159C14.1
AC225024.1 (GenBank)
[View]
[Download fasta]
[Download full submission] (275.67 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (30)
1111, 1153, 1248, 1374, 1437, 1589, 1771, 1896, 1981, 2004, 2270, 2418, 2418, 2495, 2535, 2785, 3155, 3155, 3738, 4743, 5061, 5321, 5321, 5743, 6060, 6256, 6724, 7477, 11154, 13412
HindIII in silico (32)
1012, 1041, 1097, 1168, 1232, 1362, 1436, 1608, 1763, 1871, 1963, 1985, 2270, 2409, 2440, 2505, 2559, 2778, 3111, 3166, 3739, 4621, 4863, 5007, 5109, 5420, 5778, 6044, 6625, 7474, 11055, 13464
Fragment Lengths Match Score
0.96
Annotations  
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LE_HBa0159C14 has no browsable sequence annotations
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