Clone hba-168f14

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Clone
Clone name
hba-168f14
LE_HBa0168F14
C09HBa0168F14
P168F14
Clone type
BAC
Contamination
none
Estimated length
82,075
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg3456 : [coherent], 1 members:
C09HBa0168F14
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
Brix9-2-5Tomato-EXPEN 2000plate 13F5
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 9
FISH map 
View on FISH map chromosome 9
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
123-03 1CompositeFluorescencePhase81.0%
123-04 1CompositeFluorescencePhase75.2%
123-05 1CompositeFluorescencePhase79.3%
123-08 1CompositeFluorescencePhase79.5%
123-09 1CompositeFluorescencePhase78.3%
123-21 1CompositeFluorescencePhase73.8%
Mean±sd77.9%±2.8%

Given an estimated length of 5.4µm for chromosome 9P and the mean percentage above, this BAC is approximately 4.2µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 9 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 9
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'spain'
Full Sequence
116,478 bp
C09HBa0168F14.3
EF647606.2 (GenBank) 		[View]
[Download fasta]
[Download full submission] (532.13 MB)
End Sequences
Warnings
  • poor correspondence with in vitro restriction fragments
    (match score below 0.7)
  • sequence length 116,478 is 41.9% different from estimated length 82,075
 FTP
Chromosome 9 Sequencing repository (FTP)
Chromosome Assembly
View on AGP map chromosome 9
  
Restriction Fragments
HindIII in vitro (14)
2356, 2842, 3392, 4062, 4252, 4705, 4787, 5412, 5412, 6099, 7631, 8756, 11028, 11341
HindIII in silico (29)
1032, 1043, 1053, 1192, 1430, 1493, 1597, 1681, 1703, 2089, 2158, 2269, 2369, 2389, 2834, 2835, 3415, 4135, 4270, 4626, 4685, 5110, 5131, 5288, 5901, 7522, 8692, 10682, 11066
Fragment Lengths Match Score
0.66
Annotations  
Browse
LE_HBa0168F14 has no browsable sequence annotations
Download
 
User commentsNone[Add comment] 
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End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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