Clone hba-6e18

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Clone
Clone name
hba-6e18
LE_HBa0006E18
C04HBa0006E18
P006E18
Clone type
BAC
Contamination
none
Estimated length
125,909
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg1173 : [coherent], 10 members:
C04HBa0006E18, LE_HBa0164F08, LE_HBa0165L02, LE_HBa0171J15, LE_HBa0176B07, LE_HBa0190G18, LE_HBa0218E22, LE_HBa0256N02, LE_HBa0273D07, LE_HBa0336L13
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1322Tomato-EXPEN 2000plate 8B3
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 4
FISH map 
View on FISH map chromosome 4
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
175-03a 1CompositeFluorescencePhase22.2%
175-03b 1CompositeFluorescencePhase26.2%
175-03c 1CompositeFluorescencePhase23.4%
175-04 1CompositeFluorescencePhase22.8%
175-06 1CompositeFluorescencePhase26.6%
175-08 1CompositeFluorescencePhase27.8%
175-11 1CompositeFluorescencePhase26.0%
175-17 1CompositeFluorescencePhase24.2%
175-19 1CompositeFluorescencePhase25.5%
175-20 1CompositeFluorescencePhase23.2%
175-21 1CompositeFluorescencePhase22.6%
175-25 1CompositeFluorescencePhase23.3%
Mean±sd24.5%±1.8%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 3.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 4 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 4
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
120,037 bp
C04HBa0006E18.1
CT990638.4 (GenBank) 		[View]
[Download fasta]
[Download full submission] (0.08 MB)
End Sequences
Warnings
none
FTP
Chromosome 4 Sequencing repository (FTP)
Chromosome Assembly
View on AGP map chromosome 4
  
Restriction Fragments
HindIII in vitro (32)
1025, 1205, 1205, 1313, 1450, 1515, 1586, 1665, 1665, 2263, 2318, 2441, 2607, 2972, 3069, 3294, 3333, 3441, 3598, 3770, 3770, 3770, 4076, 4310, 6476, 6476, 6836, 7648, 8490, 8490, 9299, 10533
HindIII in silico (30)
1026, 1193, 1204, 1309, 1443, 1513, 1578, 1645, 1671, 2277, 2350, 2473, 2621, 2951, 3063, 3282, 3340, 3463, 3646, 3801, 3803, 4085, 4301, 6354, 6446, 6842, 8289, 8437, 9217, 10213
Fragment Lengths Match Score
0.96
Annotations  
Browse
LE_HBa0006E18 has no browsable sequence annotations
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Seq. Center Reported Status
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 and  bp
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e.g. AP009318.1 or AP009318
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Annotations
End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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