Clone hba-13b20

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Clone
Clone name
hba-13b20
LE_HBa0013B20
C10HBa0013B20
P013B20
Clone type
BAC
Contamination
none
Estimated length
133,131
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg3959 : [coherent], 3 members:
C10HBa0013B20, LE_HBa0056G15, LE_HBa0099A21
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
447-02a 1CompositeFluorescencePhase96.3%
447-05a 1CompositeFluorescencePhase97.1%
447-05b 1CompositeFluorescencePhase96.6%
447-11 1CompositeFluorescencePhase97.2%
447-17 1CompositeFluorescencePhase100.0%
447-19 1CompositeFluorescencePhase96.6%
447-24a 1CompositeFluorescencePhase95.5%
447-24b 1CompositeFluorescencePhase94.5%
447-24c 1CompositeFluorescencePhase95.9%
447-30 1CompositeFluorescencePhase95.5%
447-33 1CompositeFluorescencePhase96.3%
427-34 1CompositeFluorescencePhase94.8%
427-37 1CompositeFluorescencePhase93.6%
427-39 1CompositeFluorescencePhase94.9%
447-42 1CompositeFluorescencePhase95.2%
427-43 1CompositeFluorescencePhase93.8%
427-44 1CompositeFluorescencePhase97.3%
427-45 1CompositeFluorescencePhase95.4%
447-49 1CompositeFluorescencePhase98.0%
correction 21.0%
Mean±sd91.3%±1.5%

Given an estimated length of 10.1µm for chromosome 10Q and the mean percentage above, this BAC is approximately 9.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

2The position of this BAC has been adjusted slightly to reflect its location relative to the typical position of marker BAC LE_HBa0234C10.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 10 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 10
HTGS phase
3 - finished
Sequencing Status
in_progress
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
126,502 bp
C10HBa0013B20.2
AC233123.5 (GenBank)
[View]
[Download fasta]
[Download full submission] (0.08 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (35)
1056, 1116, 1307, 1339, 1339, 1438, 1494, 1699, 1798, 2171, 2289, 2540, 2667, 2667, 2723, 2813, 3153, 3213, 3504, 3673, 3673, 3756, 3861, 3966, 4102, 4723, 4817, 5369, 6045, 6167, 6740, 7569, 7856, 7856, 12632
HindIII in silico (37)
1033, 1061, 1103, 1171, 1233, 1294, 1304, 1334, 1335, 1445, 1502, 1706, 1792, 2181, 2306, 2556, 2683, 2684, 2766, 2822, 3163, 3231, 3537, 3710, 3753, 3832, 3897, 4024, 4164, 4604, 4691, 5072, 5808, 5815, 6757, 7787, 12772
Fragment Lengths Match Score
0.96
Annotations  
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LE_HBa0013B20 has no browsable sequence annotations
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