Clone hba-216m19

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Clone
Clone name
hba-216m19
LE_HBa0216M19
C08HBa0216M19
P216M19
Clone type
BAC
Contamination
none
Estimated length
136,029
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2046 : [coherent], 1 members:
C08HBa0216M19
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
58-1 1CompositeFluorescencePhase85.3%
58-13 1CompositeFluorescencePhase86.8%
58-17 1CompositeFluorescencePhase78.4%
58-18 1CompositeFluorescencePhase86.0%
58-19 1CompositeFluorescencePhase85.1%
58-20 1CompositeFluorescencePhase87.4%
58-5 1CompositeFluorescencePhase86.9%
58-8a 1CompositeFluorescencePhase91.4%
58-8b 1CompositeFluorescencePhase85.8%
Mean±sd85.9%±3.4%

Given an estimated length of 4.9µm for chromosome 8P and the mean percentage above, this BAC is approximately 4.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 8 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 8
HTGS phase
none
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
164,422 bp
C08HBa0216M19.1
[View]
[Download fasta]
[Download full submission] (453.20 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (27)
1088, 1167, 1261, 1261, 1406, 1406, 2221, 2440, 2440, 2549, 2690, 2690, 2841, 3036, 3537, 3696, 3928, 4282, 5320, 6916, 7903, 8698, 8698, 9398, 13295, 13295, 18567
HindIII in silico (37)
1072, 1109, 1216, 1393, 1406, 1467, 1556, 1698, 1840, 1896, 2072, 2225, 2391, 2480, 2560, 2582, 2679, 2703, 2708, 2730, 2753, 2821, 2873, 3005, 3573, 3690, 3927, 4268, 5007, 6804, 7779, 8458, 8470, 9276, 12834, 13048, 19769
Fragment Lengths Match Score
0.84
Annotations  
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LE_HBa0216M19 has no browsable sequence annotations
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