Clone Read LE_HBa0049I14_T7_10011

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0049I14_T7_10011
Primer
T7
ID from Seq. Facility
LE_HBa_049_I14-T7
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
825 trimmed for vector and quality, 923 raw
Genbank Accession
Sequence
>LE_HBa0049I14_T7_10011 (raw untrimmed)
CCTAAACTGGTACCGGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAATACAAAAACTAATTCTTAAACTTAGTTGATAGACTCGTAAGTTT
TTCTTGTGTGTTCACAACCATCAGTTGACAGACAATTTTGCTAAGCACCGATCCTCCCTCTTTATTTGTACAAGATTTCACTTATTCTATGATCTCTCAG
GACTGGTCATTCCTGAATATTTTCATGAGACTTCTCTTATCCTATGTCATCTTAAGACTTACACAACAATGATTATAAAGTAACAATTGCATTTCTTAAA
TGGATTGGTATCCATACAGATCTCAATTCAACTTTTCCTAAGTTTTACAAAGAAAATCAACAATATCCAACCCAAATATTAACTTTCCTATAACACAAAT
TATGCCTACAAAACAAAATAGAGTAAAACGTAGATTTAGAGTGACAAAAGTGCTCCACATAAAACTCAAAAACAACAATTTAACGTTACCAAATGTAGCA
AGTAAGGTTTAACCAAAAAGATTTACAGAAAATTTCACTTATCAATTTGAAAAGCTTTGGAGATTAGATTTTTTCAGTTTAGCAGGCCTCAAAAAGTTGC
TTACGACACAACAGTAAATCAGAAAAATGAAACAATGAGTTTTCTTTTAGAAAAAGAAAACCACAACCACCCCCTGCTCAAATCACACAGAGACAGAGAG
AACAAAACTCTAACTTGGAATCAAATTAATCAATAGCCAAATTTAACTAAAAATGAAACCTTAGAATTCAACTTCCCCTAAATTCCACAAAGAAAATCAA
CAACATTCAACCCAAACTGTAATTTTCATACAACACAAATTATACCTATTAAATAAAGAGTNAAACGTAGATTTAGAGTGACAATAAATGCTCCACATCA
AAACTCAAAAGCAACAGAACAAA
>LE_HBa0049I14_T7_10011 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTAATACAAAAACTAATTCTTAAACTTAGTTGATAGACTCGTAAGTTTTTCTTGTGTGTTCACAACCATCA
GTTGACAGACAATTTTGCTAAGCACCGATCCTCCCTCTTTATTTGTACAAGATTTCACTTATTCTATGATCTCTCAGGACTGGTCATTCCTGAATATTTT
CATGAGACTTCTCTTATCCTATGTCATCTTAAGACTTACACAACAATGATTATAAAGTAACAATTGCATTTCTTAAATGGATTGGTATCCATACAGATCT
CAATTCAACTTTTCCTAAGTTTTACAAAGAAAATCAACAATATCCAACCCAAATATTAACTTTCCTATAACACAAATTATGCCTACAAAACAAAATAGAG
TAAAACGTAGATTTAGAGTGACAAAAGTGCTCCACATAAAACTCAAAAACAACAATTTAACGTTACCAAATGTAGCAAGTAAGGTTTAACCAAAAAGATT
TACAGAAAATTTCACTTATCAATTTGAAAAGCTTTGGAGATTAGATTTTTTCAGTTTAGCAGGCCTCAAAAAGTTGCTTACGACACAACAGTAAATCAGA
AAAATGAAACAATGAGTTTTCTTTTAGAAAAAGAAAACCACAACCACCCCCTGCTCAAATCACACAGAGACAGAGAGAACAAAACTCTAACTTGGAATCA
AATTAATCAATAGCCAAATTTAACTAAAAATGAAACCTTAGAATTCAACTTCCCCTAAATTCCACAAAGAAAATCAACAACATTCAACCCAAACTGTAAT
TTTCATACAACACAAATTATACCTA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.922621
Expected error %
0.236658
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (3.76%) Frame 2
pT7T3D cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13869.1:118-318 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (3.76%) Frame 2
pT7T318U cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0049I14
Clone
Clone name
hba-49i14
LE_HBa0049I14
P049I14
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found