Clone Read LE_HBa0158N21_SP6_103192

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0158N21_SP6_103192
Primer
SP6
ID from Seq. Facility
LE_HBa_158_N21-SP6
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
53 trimmed for vector and quality, 817 raw
Sequence
>LE_HBa0158N21_SP6_103192 (raw untrimmed)
CCAAGCATCGCTTGCGCAATAAAGATATCAGTATATCGCATTAAGCTCGAAGTAGATAGACCTCTTCTTAATTTCAGCGTTTAAGGGGAGTNGGCCTTGC
AATAACAAATGATGGATACTCTTAATGTTACGCTACGTATGTGTCAGTGTTGTACAAGTGTTCGGTCAAGTCTCGTAAACCATTAGAAAGGCACTGAAGA
CGGCGTTATATGTGAAAAAAGTG
AGGAAGTTTTGAGCTTTTGCGCTTTCGTCTAGAATGGAACTCATAGATTCTGGAGAAAACTGTCTGATTCATTAGCG
TTCCTAGAGGATGTCGGCATGCTTCGTGTTATGGTCAGGCACCATTAATGGAGTGATCTCGTGACACATGGAAGGCAAGTGCAAGTGTTGCAGCGAGTGA
AGTGAACTATATAGGTCCTCTGCTCTTTCCTCCCCTTCGAGATCATCCNTCAACCTATCGTACCAGTTAGTGAATAATATACATCTCTTTATACCGCGCT
TGTATTCGATTTATTAATTCACATTCTCCCATCATCGTTTCTTTTTGTTCATGATCGATCACATCCTCTAATATTCTCTGGATTATACTCCTCTACCATC
CTCTGCATAGTGAACTTTCATAGTTCTTCATGTTTTGTTGTCCCTTGTATATGACTGTTTCATCATGCTCGCCACTACGTACTACCTCTTATATAAGTCA
AATTCTCCAAGGCCGTCTCTACGCTTACCATGTATCAGTGACACATCGAGCGCTGTTATTAGTGCATAAATACACAGTTACATATTGCTACTACACTATT
TCACTCAACTTCTACTC
>LE_HBa0158N21_SP6_103192 (trimmed for quality and cloning vector)
AGGAAGTTTTGAGCTTTTGCGCTTTCGTCTAGAATGGAACTCATAGATTCTGG

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Low complexity
Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.810934
Expected error %
1.56923
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0158N21
Clone
Clone name
hba-158n21
LE_HBa0158N21
P158N21
Clone type
BAC
Contamination
none
Estimated length
111,245
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found