Clone Read LE_HBa0050C06_T7_10475

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0050C06_T7_10475
Primer
T7
ID from Seq. Facility
LE_HBa_050_C06-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
769 trimmed for vector and quality, 827 raw
Genbank Accession
DU029448
Sequence
>LE_HBa0050C06_T7_10475 (raw untrimmed)
CTGGGGACCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTCAGGAAAGTATTAGTAAAATCTCACCAGAAGCTATGCGATGCTCAACCACT
TGCTCTTCAAGACAAAACAATGGCAATCTAGCGAGTCGGAAAAGAAAGTTGAGCTATTATCAAGCAACTTCCCTGCCAGTTATAAATTTAGAAAACGAAT
TCTCCCGACATTCTACAGCTGCTGGTAAAAACTTGCATCTTTTGGAAGAAGCAGCTGAGAATGTAGTTGGTGATCCATTTAATGATGACCTGTTTTTTCA
AAGTATCGATTTTGATGCAGTGGAAGAAGAGGCTACTAGAATGCTTAGAAACAAATCACAGTCCTTGGTTCAAAATACAGTGACCTCAATACCTACTACT
CAAATTTCTAATGGCACAGACGCTCCTTCATTTGATCTGGGAATTTAGTGTTGCTGTCTTAACCGTCAATTTTTGTATGACAGCGTTCATGATCATTTAT
GTAAAGGAAAAACATATTAAGTTATTATCTGTGCAGAATAGAATAGTTGTATTTATCATTTAATAGGTTATAGGAAAGTCTATCTTGATGAAACTGACTG
TATTTGCTAGGAGTGTGGAAATATTTTGATTGTTTCCAAAATGTATATAATCTCACAATTAGTGTTCCCTTGGTCCAAGTAAAGTTTTGAGGCTTTTGTT
AATAGCTGTGTTTTCTCAAGATGGTATGTTTCTCTTATTTGTTACGAGTACCTTAATGTTTTAAGGATTTGTATATTGGGGTAACAACGGAATTGGATAT
GAGTTGATAGAAAATGAGTTAGGAGAA
>LE_HBa0050C06_T7_10475 (trimmed for quality and cloning vector)
AGCTTTCAGGAAAGTATTAGTAAAATCTCACCAGAAGCTATGCGATGCTCAACCACTTGCTCTTCAAGACAAAACAATGGCAATCTAGCGAGTCGGAAAA
GAAAGTTGAGCTATTATCAAGCAACTTCCCTGCCAGTTATAAATTTAGAAAACGAATTCTCCCGACATTCTACAGCTGCTGGTAAAAACTTGCATCTTTT
GGAAGAAGCAGCTGAGAATGTAGTTGGTGATCCATTTAATGATGACCTGTTTTTTCAAAGTATCGATTTTGATGCAGTGGAAGAAGAGGCTACTAGAATG
CTTAGAAACAAATCACAGTCCTTGGTTCAAAATACAGTGACCTCAATACCTACTACTCAAATTTCTAATGGCACAGACGCTCCTTCATTTGATCTGGGAA
TTTAGTGTTGCTGTCTTAACCGTCAATTTTTGTATGACAGCGTTCATGATCATTTATGTAAAGGAAAAACATATTAAGTTATTATCTGTGCAGAATAGAA
TAGTTGTATTTATCATTTAATAGGTTATAGGAAAGTCTATCTTGATGAAACTGACTGTATTTGCTAGGAGTGTGGAAATATTTTGATTGTTTCCAAAATG
TATATAATCTCACAATTAGTGTTCCCTTGGTCCAAGTAAAGTTTTGAGGCTTTTGTTAATAGCTGTGTTTTCTCAAGATGGTATGTTTCTCTTATTTGTT
ACGAGTACCTTAATGTTTTAAGGATTTGTATATTGGGGTAACAACGGAATTGGATATGAGTTGATAGAA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.95846
Expected error %
0.323526
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g35530.1 Score 74 E Value 9e-14 Identity 14.60% Align. Length 389bp (50.59%) Frame 2
68414.m04411 DEAD/DEAH box helicase, putative low similarity to RNA helicase/RNAseIII CAF protein [Arabidopsis thaliana] GI:6102610; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_174785.1| Score 74 E Value 4e-12 Identity 14.60% Align. Length 389bp (50.59%) Frame 2
DEAD/DEAH box helicase, putative [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF79368.1| Score 74 E Value 4e-12 Identity 14.60% Align. Length 389bp (50.59%) Frame 2
F15O4.40 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0050C06
Clone
Clone name
hba-50c6
LE_HBa0050C06
P050C06
Clone type
BAC
Contamination
none
Estimated length
126,590
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found