Clone Read LE_HBa0051L15_T7_11693

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0051L15_T7_11693
Primer
T7
ID from Seq. Facility
LE_HBa_051_L15-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
912 trimmed for vector and quality, 959 raw
Genbank Accession
DU030548
Sequence
>LE_HBa0051L15_T7_11693 (raw untrimmed)
TAAGTCGGTAACCGGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCGAAAAGGTATTAAAACCCCTGCCCAGAACCCTATATATTGCCAAGTG
TCAAGAACAAATTCACCAGGAAACACGAAATAAAAGGTGGATGCCTCCTAAAAAGTAAGACATGGCCAAAGCAAATGTACACTCATGCTATAAGATAAAA
AGAAAAGCTTCTGCCACAGACAGCTGTTATAGTGACAAGAGCACATACAAACTCAACTTTAAGTTTCCAAAGCTGAAAGAGAACTTACCCATGCAGGAAA
CCATTCTGGCAAAGTAAAATATTCTGGTTTAAACAGTGGGCCAGGGTCACAATCAAGCCAACCAGTTGATAGGTCCTGCATCACAAAATATGGAAGTTAA
GCTTTCAAAACTGAGCAGCTAGTCTCCCAAGCCAATAGAGAAACTTTACCAGAATTACCTTTCTAGGGCATGTCAGCCACTGAAAATGACCAAACATATT
CGCAAGCTGAAAAAACAACAGATGATAAGCAAATCTATAAAAAGAGAAAAAAACATATACATTTCTCAAATACTGTTCAGAAGAAAAATCTTTATCATAT
TTCTATTAACATACATAAAAACTACAATACAATTTTAAGCATGAGAACTTAGATGAGTCTGTCTTGAGAAGGTAGCATAACCACATAAGAGTAGGATTAA
AACTACCCCAACTATGCAATCATTCCAAAATGGAGGAAAGACTAATGCACTACACTGGAAGTATAAGTGTATTGCAAATCTGCAATTGCTATTGCCCCAA
ATAAAAGTTACCAGAAAGGCAGAGATAATGGTTGTAATAGATGCTCCAATGAAGCCTTCCCAAGTTTTCTTAGGGGACAATTTGATCAAAGGAGTTCGTC
CAAAGAGGAATCCGAAAAATAGGCAGCTATATCATTGATCACGATGAGTGAACCTGGAA
>LE_HBa0051L15_T7_11693 (trimmed for quality and cloning vector)
AGCTTCGAAAAGGTATTAAAACCCCTGCCCAGAACCCTATATATTGCCAAGTGTCAAGAACAAATTCACCAGGAAACACGAAATAAAAGGTGGATGCCTC
CTAAAAAGTAAGACATGGCCAAAGCAAATGTACACTCATGCTATAAGATAAAAAGAAAAGCTTCTGCCACAGACAGCTGTTATAGTGACAAGAGCACATA
CAAACTCAACTTTAAGTTTCCAAAGCTGAAAGAGAACTTACCCATGCAGGAAACCATTCTGGCAAAGTAAAATATTCTGGTTTAAACAGTGGGCCAGGGT
CACAATCAAGCCAACCAGTTGATAGGTCCTGCATCACAAAATATGGAAGTTAAGCTTTCAAAACTGAGCAGCTAGTCTCCCAAGCCAATAGAGAAACTTT
ACCAGAATTACCTTTCTAGGGCATGTCAGCCACTGAAAATGACCAAACATATTCGCAAGCTGAAAAAACAACAGATGATAAGCAAATCTATAAAAAGAGA
AAAAAACATATACATTTCTCAAATACTGTTCAGAAGAAAAATCTTTATCATATTTCTATTAACATACATAAAAACTACAATACAATTTTAAGCATGAGAA
CTTAGATGAGTCTGTCTTGAGAAGGTAGCATAACCACATAAGAGTAGGATTAAAACTACCCCAACTATGCAATCATTCCAAAATGGAGGAAAGACTAATG
CACTACACTGGAAGTATAAGTGTATTGCAAATCTGCAATTGCTATTGCCCCAAATAAAAGTTACCAGAAAGGCAGAGATAATGGTTGTAATAGATGCTCC
AATGAAGCCTTCCCAAGTTTTCTTAGGGGACAATTTGATCAAAGGAGTTCGTCCAAAGAGGAATCCGAAAAATAGGCAGCTATATCATTGATCACGATGA
GTGAACCTGGAA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.942073
Expected error %
0.735076
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g62430.1 Score 73 E Value 1e-14 Identity 74.40% Align. Length 668bp (73.25%) Frame 3
68414.m07043 phosphatidate cytidylyltransferase / CDP-diglyceride synthetase (CDS1) identical to SP|O04928 Phosphatidate cytidylyltransferase (EC 2.7.7.41) (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase) {Arabidopsis thaliana}
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g22340.1 Score 72 E Value 2e-14 Identity 85.00% Align. Length 163bp (17.87%) Frame 1
68417.m03230 phosphatidate cytidylyltransferase, putative / CDP-diglyceride synthetase, putative similar to CDP-diacylglycerol synthetase (CDS) GI:2181182 from [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA63004.1| Score 77 E Value 3e-18 Identity 91.50% Align. Length 677bp (74.23%) Frame 3
CDP-diacylglycerol synthetase [Solanum tuberosum] pir||T07366 probable phosphatidate cytidylyltransferase (EC 2.7.7.41) CDS - potato sp|O04940|CDS1_SOLTU Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA63969.1| Score 73 E Value 6e-13 Identity 82.90% Align. Length 163bp (17.87%) Frame 1
CDP-diacylglycerol synthetase [Arabidopsis thaliana] gb|AAF70845.1| F24O1.17 [Arabidopsis thaliana] ref|NP_176433.2| phosphatidate cytidylyltransferase / CDP-diglyceride synthetase (CDS1) [Arabidopsis thaliana] sp|O04928|CDS1_ARATH Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0051L15
Clone
Clone name
hba-51l15
LE_HBa0051L15
P051L15
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found