Clone Read LE_HBa0028H07_T7_138194

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0028H07_T7_138194
Primer
T7
ID from Seq. Facility
LE_HBa_028_H07-T7
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
672 trimmed for vector and quality, 999 raw
Genbank Accession
Sequence
>LE_HBa0028H07_T7_138194 (raw untrimmed)
GGGAAACTGGTCCGGGGGGTATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATTCCATCAAAGGTTATGTGGTTTGTTGAAAAGCCCATTGCAAAACC
ACCACACTTAAATGATGTAACCTAACCAAAAAGTAATCAATTAAATGTTTATAGCTTTACATTGTCAAATTTCCTATAAGATAACTACAAATAACTGTCC
ACAAGAGAATAAATCAACAATGTATAAAATAATAAAAAAAGTTACATACGAAAACTTACAACAAGTTAAAATATAAAAATAATAAAAAGTTTCTTTGAAA
AGAAAGATAGATTTAGGGTGAATTCAATGAGTTCAACCGAATACGTTATTCTCGATTCAGTCTATTTATTTATATATATATATATATATATATATATATA
TATATATATATTAGATTTATGCATGTCTGTCATTAATATTTGTGTTTATTAATTTTTATAAAGTACAGCTAGGTAGTAGGTGAAGGAATAATTATTTGAA
AGGTACATATGAACCCTCTATTTGTCCTATGAAAAAAGTTAAAATGGCTAAATAGCATATACTAGTATATAGAATATAGTATAGTATTAATAAGTGACAA
TTTTGTGTACTTTTACTTTCTAACTAGTGTTTGAACTTAAATCTAAAGACTAGCAATTACCTATCAAAAGTTATCGTTGAGTGATAAAGTATTCTTTTTT
TAAAATTAAAGTTCTCAGGTTTAAGCACTAAAATAAACTAATCGTCTTTGGTAAAGATAAAGTTACCTCTAGAAGTAGGATTTCCTCATATGAAACCAAT
TTAGTTAAGCTTCAAACTGAGTATCAAACGCTGAATCAAAAAAAATAAAAAATTTCCCTAGTGTAATTGGTATGGAAAAAATTGGTCCAATATTATTTAT
TATAAAATAAAAATGGAAAAATTTTATTTATTTTTTATCTGAAGGAGCCAAAAATTGAATTTCGGAAATCCCGTTCTTAGTCTTTGGGTTTTGAATTTA
>LE_HBa0028H07_T7_138194 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTATTCCATCAAAGGTTATGTGGTTTGTTGAAAAGCCCATTGCAAAACCACCACACTTAAATGATGTAACC
TAACCAAAAAGTAATCAATTAAATGTTTATAGCTTTACATTGTCAAATTTCCTATAAGATAACTACAAATAACTGTCCACAAGAGAATAAATCAACAATG
TATAAAATAATAAAAAAAGTTACATACGAAAACTTACAACAAGTTAAAATATAAAAATAATAAAAAGTTTCTTTGAAAAGAAAGATAGATTTAGGGTGAA
TTCAATGAGTTCAACCGAATACGTTATTCTCGATTCAGTCTATTTATTTATATATATATATATATATATATATATATATATATATATATTAGATTTATGC
ATGTCTGTCATTAATATTTGTGTTTATTAATTTTTATAAAGTACAGCTAGGTAGTAGGTGAAGGAATAATTATTTGAAAGGTACATATGAACCCTCTATT
TGTCCTATGAAAAAAGTTAAAATGGCTAAATAGCATATACTAGTATATAGAATATAGTATAGTATTAATAAGTGACAATTTTGTGTACTTTTACTTTCTA
ACTAGTGTTTGAACTTAAATCTAAAGACTAGCAATTACCTATCAAAAGTTATCGTTGAGTGATAAAGTATTC

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.90299
Expected error %
0.0405
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 72 E Value 8e-14 Identity 100.00% Align. Length 35bp (5.21%) Frame 2
pT7T3D cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13869.1:118-318 Score 72 E Value 8e-14 Identity 100.00% Align. Length 35bp (5.21%) Frame 2
pT7T318U cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0028H07
Clone
Clone name
hba-28h7
LE_HBa0028H07
C11HBa0028H07
P028H07
Clone type
BAC
Contamination
none
Estimated length
52,764
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found