Clone Read LE_HBa0079M24_T7_20207

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0079M24_T7_20207
Primer
T7
ID from Seq. Facility
LE_HBa_079_M24-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
730 trimmed for vector and quality, 907 raw
Genbank Accession
DU037378
Sequence
>LE_HBa0079M24_T7_20207 (raw untrimmed)
AGCTCCTGGGCCGGGGGGACTTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGGCAGATGAGAAGAGATCACCCTTTTTTTTTTGTCGTTTGATGGGATT
TGAACCCGAAATCTTATCGTTCTCCCCTATTATATTAACCACTTGGCCACAACCGTGGGTTCAAAGATACATGGTTGTTTTGGCGCAAAAAAGTTTGATT
TATTTGGTTCTTGGTGTCTATAGTGCCTTTTACTAGTTTTCATTTGACATACTTCATTCTTATAGATGCTATTGTAATTGTGCTTAGATTTCCTATTTCT
TAAACAAGATTAAAAAAGAAAAATTTGAGTTATATTTGAAGATCAAAGAAATATTTTAAGGTTTTGAACTAATCTATATAAATGTTAATATGAAATTAGA
ACAAATGACGCACCCAAGTGTGTAGCCTAGTGAGTTGAGAACCATGAGGTCTTAAGTTCAAATTCCAGCCGAGACAAAAAAAGTACTGGGTGATTTCTTC
CTATGCGTCCTAACCTTGGTGGATAGAGTCACTTGTTACTTGGTGGTGGGAGTCAAAGTATCTCCTGGAATTAGTCGAGGTGTGCGCTAGCTGGTCCGGA
ACACCACGGTTATTTAAAAAAATTAGAATAAATGACGTATGAATGTTAAATTAGTAAGGTTAGGTAAAATTAACACTGTACCAATATTTGATTGTTGTGG
CCATAACTCCCCTCTCCCCCCAACCTTCTCTCTGCAGATGGAGGTATTGTCCTCTTGAAAATGACACAAGCAAATGAATGCATTAAAGAATCTGTAGAAG
CAGTTTTGAACGTAAAAGGACTAAAAAAATTGAAAGGAATTGGTAAATCCACAAAAAAAGAAAAAGAATTTGTGACTGAAACCAGGGGAATTGTAGCCAA
CTTTTTG
>LE_HBa0079M24_T7_20207 (trimmed for quality and cloning vector)
TAGAGTCGACCTGCAGGCATGCAAGCTTGGGCAGATGAGAAGAGATCACCCTTTTTTTTTTGTCGTTTGATGGGATTTGAACCCGAAATCTTATCGTTCT
CCCCTATTATATTAACCACTTGGCCACAACCGTGGGTTCAAAGATACATGGTTGTTTTGGCGCAAAAAAGTTTGATTTATTTGGTTCTTGGTGTCTATAG
TGCCTTTTACTAGTTTTCATTTGACATACTTCATTCTTATAGATGCTATTGTAATTGTGCTTAGATTTCCTATTTCTTAAACAAGATTAAAAAAGAAAAA
TTTGAGTTATATTTGAAGATCAAAGAAATATTTTAAGGTTTTGAACTAATCTATATAAATGTTAATATGAAATTAGAACAAATGACGCACCCAAGTGTGT
AGCCTAGTGAGTTGAGAACCATGAGGTCTTAAGTTCAAATTCCAGCCGAGACAAAAAAAGTACTGGGTGATTTCTTCCTATGCGTCCTAACCTTGGTGGA
TAGAGTCACTTGTTACTTGGTGGTGGGAGTCAAAGTATCTCCTGGAATTAGTCGAGGTGTGCGCTAGCTGGTCCGGAACACCACGGTTATTTAAAAAAAT
TAGAATAAATGACGTATGAATGTTAAATTAGTAAGGTTAGGTAAAATTAACACTGTACCAATATTTGATTGTTGTGGCCATAACTCCCCTCTCCCCCCAA
CCTTCTCTCTGCAGATGGAGGTATTGTCCT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.961405
Expected error %
0.441379
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L08952.1:1-181 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.11%) Frame 2
pT7T319 cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0079M24
Clone
Clone name
hba-79m24
LE_HBa0079M24
P079M24
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found