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Clone Read LE_HBa0089A23_SP6_25004

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0089A23_SP6_25004
Primer
SP6
ID from Seq. Facility
LE_HBa_089_A23-SP6
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
5 trimmed for vector and quality, 931 raw
Sequence
>LE_HBa0089A23_SP6_25004 (raw untrimmed)
CACGGCTTAAGGGAGCTTGACAAAGGACATAAAAGAATGCTACGATTNGGAAACTATCGACTATATCTTACGCATGATATTGGGGTGGTAGGATATTAGC
ATTGTAGTCATCAGCATGAAGTAGTTCAGCCCCAATACCCTTTATCCTCCTGTGTTCATTCTCTCCAGGGACACACCAGAAATAGTAATAACTGATAGAG
CTCTCGTCTTATCACTCGTTCGAGAGATTGTCCTCTCGAGAAAAAGGCTAACAAGGGAAGAAAAAAAGAGACCCCATTCCTTCTCACAAAACAAGTAGGC
AGTGCTCGGTCCATCCTCACTGAAATCATCTGAGATACACCCTTCCTTCGCATATGGAAGAGGGACCGATGACAAACGGTTTTTTTATTATTGTCCTTTC
GCAAACTACCAAGATTCAGGATTGATTCTTCTTAAGCTTAGCTCTTTGGTTAAATTACAATGTCCTTTGATCCTCGATACGTTAAACCAGTGTCAAGAAT
CCGGGAGGAATCTTTCGTCCTTTGGGAAACTGGCCCAATACTACCTTTGATAATTTTTTACTCTGCTCCTTGAGAACTGGTTCCGTAAAAGACGGGATAA
TCTTTTTCTAAACTCCGCTGTCACCCTGTCGTTACCTCTAAGTTATTCGACGACGAAGCTCCCACCTACCATACACCGAGACAAGTGGCCGATACCATCT
GTGCCTGGCGCTCCCCCTTCATAATAACTTTGATGATACTCACCGATGTTGCTGATATGTGGACTGAATTTTTCTTCGTGTCATGCCTCCATACATGGTG
ACCTTTTTACATCCTNTCCTTCTATATATCATCGACTTACGCCCGTTTACCCACAATACTTGTCTTGTTCTTCATCCTCTCTTTCGTTCTTTTGATATCT
CATTCACACCTATAATACAATATAATAACTA
>LE_HBa0089A23_SP6_25004 (trimmed for quality and cloning vector)
TCGAC

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Low complexity
Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
1.72563
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0089A23
Clone
Clone name
hba-89a23
LE_HBa0089A23
C08HBa0089A23
P089A23
Clone type
BAC
Contamination
none
Estimated length
117,569
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found