Clone Read LE_HBa0006E15_SP6_2524

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0006E15_SP6_2524
Primer
SP6
ID from Seq. Facility
LE_HBa_006_E15-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
760 trimmed for vector and quality, 1014 raw
Genbank Accession
DU022738
Sequence
>LE_HBa0006E15_SP6_2524 (raw untrimmed)
AAAAAAAAAGGGTTTTCTTTTGCTATTGCACAGNCTCANANGAACACCANCAAAAAAAACAACGATAGCTTTGNAAGAAATCACACCAGTTTTCTGGTGA
AAAGTCTGCCAACTGCTGTTAGGCGGAAGCTTTTGAGTGAGAAGTGACTTGTATCTAAAGGCATGTACCATGAAACTGGTGAGTCTTCAGCGAGATCCTT
ATCCCACACAACCTACATATCACCGGCAAGTAGAATTTAGTGATCAAATGGCAAAGAGCAATCAAATGCTTAATAATATTATTAGAAGAAAAGGACTTGG
AACTTACTTCAAAACCAGCTTTTCTCGCTGCTTCTAGGCACTGTTGTGTTGATCTAATCTCCGGGAGACCATTTCCGAGCTCAATTTCTTCCTATGGCCA
ACAAACAACTAAGATAAGTAAGACTATCCAACATATAAACAAATGCACGGAGACAGTCCAATAAATTGATAAATGACTTCAAAACTTCAAGATTGACCTT
GATTCTTTTCTGCTCTTCATTATTTGGATTGTATGCATCAGTCATGCACCACTCATACACAGCAAAGCACTGACCAGGCTTCAGCACCCTATAGATCTCT
CTATAGCATCCAACCTGACAAAAATCAGATTTATTAAAACAAATTAGAAGAATACAATTGTATAAATCATGTCATGCACCGCAGCATATACATAGCAAAG
CAAGTGGTTTATAAAAAACGAAGTCATAATTGAACACGGTAAAGATATACACACCGGATCTGGTGCATGGCAGGTAGCTTCTATTGCATATACTGCATCA
AAGCTATTGTCAGGGAATGGCATTTTCATGAAATCACCCTGCATAGACACTTTATCAAGCACTGCACAGGCAGTTCAAAAAAGATTGACCTGATGTAACA
GTTACTACCCTTACAAAGTTGCAAGTTTGATCAATCCCCTTTGGGGTTCAACACCGCAGAGTAAAGGAGTAAACACTACTCGGAAAAATAAATTCAACCT
TTAAAAGGAACAAT
>LE_HBa0006E15_SP6_2524 (trimmed for quality and cloning vector)
AAAAAAACAACGATAGCTTTGNAAGAAATCACACCAGTTTTCTGGTGAAAAGTCTGCCAACTGCTGTTAGGCGGAAGCTTTTGAGTGAGAAGTGACTTGT
ATCTAAAGGCATGTACCATGAAACTGGTGAGTCTTCAGCGAGATCCTTATCCCACACAACCTACATATCACCGGCAAGTAGAATTTAGTGATCAAATGGC
AAAGAGCAATCAAATGCTTAATAATATTATTAGAAGAAAAGGACTTGGAACTTACTTCAAAACCAGCTTTTCTCGCTGCTTCTAGGCACTGTTGTGTTGA
TCTAATCTCCGGGAGACCATTTCCGAGCTCAATTTCTTCCTATGGCCAACAAACAACTAAGATAAGTAAGACTATCCAACATATAAACAAATGCACGGAG
ACAGTCCAATAAATTGATAAATGACTTCAAAACTTCAAGATTGACCTTGATTCTTTTCTGCTCTTCATTATTTGGATTGTATGCATCAGTCATGCACCAC
TCATACACAGCAAAGCACTGACCAGGCTTCAGCACCCTATAGATCTCTCTATAGCATCCAACCTGACAAAAATCAGATTTATTAAAACAAATTAGAAGAA
TACAATTGTATAAATCATGTCATGCACCGCAGCATATACATAGCAAAGCAAGTGGTTTATAAAAAACGAAGTCATAATTGAACACGGTAAAGATATACAC
ACCGGATCTGGTGCATGGCAGGTAGCTTCTATTGCATATACTGCATCAAAGCTATTGTCA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.968632
Expected error %
0.590374
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g13710.1 Score 77 E Value 2e-36 Identity 71.10% Align. Length 518bp (68.16%) Frame 3
68418.m01596 sterol 24-C-methyltransferase, putative similar to SP:P25087 Sterol 24-C-methyltransferase, Delta(24)-sterol C- methyltransferase, Saccharomyces cerevisiae
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAC34951.1| Score 87 E Value 7e-43 Identity 84.50% Align. Length 521bp (68.55%) Frame 3
S-adenosyl-methionine-sterol-C- methyltransferase [Nicotiana tabacum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAC35787.1| Score 84 E Value 2e-40 Identity 79.80% Align. Length 521bp (68.55%) Frame 3
S-adenosyl-methionine cycloartenol-C24-methyltransferase [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0006E15
Clone
Clone name
hba-6e15
LE_HBa0006E15
P006E15
Clone type
BAC
Contamination
none
Estimated length
111,060
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found