Clone Read LE_HBa0125I08_T7_254026

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0125I08_T7_254026
Primer
T7
ID from Seq. Facility
LE_HBa_125_I08-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
666 trimmed for vector and quality, 975 raw
Genbank Accession
DU081610
Sequence
>LE_HBa0125I08_T7_254026 (raw untrimmed)
NNTTACTGACGGGGATCTCTGAGTCGACCTGCAGGCATGCAAGCTTGCAAGTGAGCTACACTTCCCATGCTTCTCGCCTTTCTACTTAAAGCATCCGCTA
CAACATTCGCCTTCCCCGGGTGATACAAAATAGTGATGTCGTAGTCCTTCAGTAGTTCCATCCATCTCCTCTGTCTCAAGTTCAAATCTTTCTGAGTAAC
GACATACTGTAGGCTACGATGATCCGTATAGACCTCACACTTAACCCCATATAAATAGTGTCTCCATTGCTTTAATGCAAACACTACCGTGGCCAATTCC
AAATCATGAGTTGGATAGTTACGTTCATGCTCTTTTAATTGTCTTGAAGCATAAGCAATCACATTCTTCTCTTGCATTAGCACTGCACCCAAACCCGAAC
AGGATGCATCACAATAGACGATGAAATTCTTACCTTCCACTGGCAAGGTGAGAATTGGTGCGGTAGTCAACAAAGTCTTGAGCTTCTGAAAGCTTTCTTC
ACACTCATCCGACCATACAATTGGAACATTTTGCTTAGTCAAGTTCGTCAGTTGGGAAGCAATAGAAGAGAATCCCTTGACAAATCGGCGGTAGTAGCTA
GCTAACCCAACAAAGCTCCGTATTTCTAACACGTTAGTAGGTCTTACCCAATTCTTTACTGTCTCAATCTTAGAAGGATCCACCATCACTCCATCCTTAG
AAACCACGTGCCCCAAGAAGGACACTGCATCTAGCAAAAACTCACACTTAGAGAACTTGGGATAAAGCTTTTTCTCCCTCAACATTTTCCAATACATTCT
CAAAGGCTCCTCAGGTCCCCCCTTAATTTTTAAATAATACAGTATGGTCTCCATAAATCCGATCCCAAAGAGATCCAAATTGGGCTTAAAATCCCGGTTC
ACAAACTCATGAACCCGGCAGGGGCATTCGTAAGAACAAAGGAAAACCCTTCAAATTCGAAAAGCCCAAACCGGG
>LE_HBa0125I08_T7_254026 (trimmed for quality and cloning vector)
TCGACCTGCAGGCATGCAAGCTTGCAAGTGAGCTACACTTCCCATGCTTCTCGCCTTTCTACTTAAAGCATCCGCTACAACATTCGCCTTCCCCGGGTGA
TACAAAATAGTGATGTCGTAGTCCTTCAGTAGTTCCATCCATCTCCTCTGTCTCAAGTTCAAATCTTTCTGAGTAACGACATACTGTAGGCTACGATGAT
CCGTATAGACCTCACACTTAACCCCATATAAATAGTGTCTCCATTGCTTTAATGCAAACACTACCGTGGCCAATTCCAAATCATGAGTTGGATAGTTACG
TTCATGCTCTTTTAATTGTCTTGAAGCATAAGCAATCACATTCTTCTCTTGCATTAGCACTGCACCCAAACCCGAACAGGATGCATCACAATAGACGATG
AAATTCTTACCTTCCACTGGCAAGGTGAGAATTGGTGCGGTAGTCAACAAAGTCTTGAGCTTCTGAAAGCTTTCTTCACACTCATCCGACCATACAATTG
GAACATTTTGCTTAGTCAAGTTCGTCAGTTGGGAAGCAATAGAAGAGAATCCCTTGACAAATCGGCGGTAGTAGCTAGCTAACCCAACAAAGCTCCGTAT
TTCTAACACGTTAGTAGGTCTTACCCAATTCTTTACTGTCTCAATCTTAGAAGGATCCACCATCAC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.976338
Expected error %
0.0858948
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT38745.1| Score 313 E Value 3e-84 Identity 23.50% Align. Length 641bp (96.25%) Frame 2
putative polyprotein, 3'-partial [Solanum demissum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT66771.1| Score 312 E Value 4e-84 Identity 23.70% Align. Length 641bp (96.25%) Frame 2
putative polyprotein [Solanum demissum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0125I08
Clone
Clone name
hba-125i8
LE_HBa0125I08
P125I08
Clone type
BAC
Contamination
none
Estimated length
95,667
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found