Clone Read LE_HBa0196F24_SP6_267354

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0196F24_SP6_267354
Primer
SP6
ID from Seq. Facility
LE_HBa_196_F24-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
464 trimmed for vector and quality, 929 raw
Genbank Accession
DU085006
Sequence
>LE_HBa0196F24_SP6_267354 (raw untrimmed)
TTTGGCAATTCTACTCTCATAGAGAGAGGAAGTTCCTATATACCTCCACACTGTTTTAAAACAACAAAGGTATGATTCACTATTTGAAACAGCAACTGTG
TGAGTGTCTATGGATCAGGGAAGCCTTACTCAAGTACTAAAGAAATACTAACCTCATAGCAACTAAAAGAATGTACTTTGCTAAGCAAGTTCAATAATCA
AGGGATAAAAACTAAAAACTCAAACAGCGCAGAAATCTAACACTTAAGCTATCACAGCAATAAGCATTGATTGGTAAATCACATACCTGAGTTTCCCAAG
GACAATACCAGATTCATTAGCACGATTAAGCCCAATATCTTCAGGAGATATAATCTCATATGTATCTTTGTGTTTTAACATTCCATCCTAAAGAGCATAA
AAGTAGTTTAAGAAGGAGAAGAGATTGAAAAGGAAAAACTGAAATATTTTTTCCTTTTTTTTTTGATTCACAACTGAACGGTTACAAAAAGGTGCGAATC
CAGCTTTGAGGTAAAGAAGAAACGGGAAGAGAGACATAATATACTACATATATTCTAAGAAGTTTTACTGAAAAATTTTATGCATTTGAAGATGAATCAA
AAAATCAATGCTAACAGGGAAATTAATCGAGCTCTAACTTAATATTTGGAAAATTTATCCCTATATTTTCAAATGTTAAATCCTAACCACCCCATCTAAA
ATGAAAAGGGGCATTAACCAAGTAATATTCGGAAATTTAAAAAAATTAGCTGATTTCAAATCCCGGATGGAATTTTTTTTTCTTCGTAAGGGAAATATTT
CTTGTACCCTCAGGGAAATATTTGTGCCCAAAGGGTTTTTGTTTTATCTCCTTTGTCCCCTGAGAATGGAATACTTTGGTGCTCCTTCTCCAATTCGTTT
TTTTTGGGGCTTCTATAAGGGTTTTACCA
>LE_HBa0196F24_SP6_267354 (trimmed for quality and cloning vector)
AGTTCCTATATACCTCCACACTGTTTTAAAACAACAAAGGTATGATTCACTATTTGAAACAGCAACTGTGTGAGTGTCTATGGATCAGGGAAGCCTTACT
CAAGTACTAAAGAAATACTAACCTCATAGCAACTAAAAGAATGTACTTTGCTAAGCAAGTTCAATAATCAAGGGATAAAAACTAAAAACTCAAACAGCGC
AGAAATCTAACACTTAAGCTATCACAGCAATAAGCATTGATTGGTAAATCACATACCTGAGTTTCCCAAGGACAATACCAGATTCATTAGCACGATTAAG
CCCAATATCTTCAGGAGATATAATCTCATATGTATCTTTGTGTTTTAACATTCCATCCTAAAGAGCATAAAAGTAGTTTAAGAAGGAGAAGAGATTGAAA
AGGAAAAACTGAAATATTTTTTCCTTTTTTTTTTGATTCACAACTGAACGGTTACAAAAAGGTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.936461
Expected error %
0.0352895
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAB61599.1| Score 69 E Value 5e-11 Identity 29.70% Align. Length 110bp (23.71%) Frame 2
2-isopropylmalate synthase [Lycopersicon pennellii] sp|O04974|LEU1B_LYCPN 2-isopropylmalate synthase B (Alpha-isopropylmalate synthase B) (Alpha-IPM synthetase B)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0196F24
Clone
Clone name
hba-196f24
LE_HBa0196F24
P196F24
Clone type
BAC
Contamination
none
Estimated length
39,477
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found