Clone Read LE_HBa0211A04_T7_372475

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0211A04_T7_372475
Primer
T7
ID from Seq. Facility
LE_HBa_211_A04-T7
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
24 trimmed for vector and quality, 898 raw
Sequence
>LE_HBa0211A04_T7_372475 (raw untrimmed)
NGGGTCGGCCGGGGATCTCTGAGTCGACCTGCAGGCATGCAAGCTTGGGGAGATAGATAGTCTGTTTCTGTTTCGGGAAGGAAGAACGAGCCGAATGGAC
TGAAGAAAACTATAAAGTATCTGTACCGTATGCTGAGGAAGCACATTGATGTTGAGAATTTGTTTTCCCTGAGGGAGTCCTTGCGTCTTGAGAGTGAAGA
GTTGTATGTGTCAACCGCAATGTCTCGCCTTGAAAGTCAGGATCCTACAGACTTCATATCTCCTGGCAGCAAGGAGATGGAAATTCCACTTCTGAGACCA
ATTTGTCCGATTATAATGTGAAGTTGTCTATGTTCGGTGGAACTCGCCATGATTAGCTATTCAAAGCAAACAACGACATACCCAACGTAGTCCGACCAGG
TGGGGTCTAGGGAGGGATAGAGTGTTGTACACAGACCTTACCCCTACCTAACGGAGATAGGAAGGCTGTCTTCGAAAGATCCCCGGCTCGGGAGTTTATA
TCAGAGACCATACTGAAATGAGGAGATCTGACTGCCACTAGAGGACGACTCGCATAGCATATGTGAAAAATGACACAACATCAACAAAATCTTAGTCATC
AAACCTGCAACGCCAACTCCCATAAGGTGATAAAATACACGAGGACAGACCGGCTTATGCGGCAACGACTAGCACAACATACCCAAAGTAGTTCCACTAG
TGGAATTTGGGAAGAGTACGATGTGTGCCCACATTACCCCTACCTTATGGGGATAGAGAGGCTGTTCCCGATACACCTTGCCTCAAAAGAAAAAGTTTCG
AGCAAGATTGGGGCCAACCTTCTGTGACTGATGTTTACCAGCGGGAACCAGGAACAACAGTCCATCCAAACCAATTACAAGACATTTACCTACCCCAT
>LE_HBa0211A04_T7_372475 (trimmed for quality and cloning vector)
GTCGACCTGCAGGCATGCAAGCTT

This is probably not a useful sequence.

Problems:
  • Low complexity
  • Short cloning insert
Warnings:
  • Not screened for repetitive elements
  • Not screened for contaminants
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
0.229321
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0211A04
Clone
Clone name
hba-211a4
LE_HBa0211A04
C12HBa0211A04
P211A04
Clone type
BAC
Contamination
none
Estimated length
129,433
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found