Clone Read LE_HBa0238M13_T7_399230

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0238M13_T7_399230
Primer
T7
ID from Seq. Facility
LE_HBa_238_M13-T7
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
642 trimmed for vector and quality, 881 raw
Sequence
>LE_HBa0238M13_T7_399230 (raw untrimmed)
NNNNNGGTCATAGCGCTCNACGGCCGCAGTGTGATGGATATCTGCAGAATTCGCCCTTAGAGTTTGATCCTAGGGGGGGATGAACGCTGGCGGCGTGCCT
AATACATGCTTTTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGAT
AACTTCGGGAAACCGAAGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTG
CATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTC
CTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTG
TTAGGGAAGAACAAGTACAAGAGTAACTGCTTGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGG
TGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAA
ACGGGGGAACTTGAGTGCAGAAGGGAAAAGCGGAATTCACCGTGTACCGGTGAAATGCCTAAAAATGTGGAGGAAACCCAATGCCGGAAGCCGGTTTTTT
GGTCTGTACTGCCCCTGAGGCCCAAAGCTGGGGGAACAAACGGAATAAAATCCCGGGTAGTCCCGCCCGTAACAAGAATGG
>LE_HBa0238M13_T7_399230 (trimmed for quality and cloning vector)
GATGGATATCTGCAGAATTCGCCCTTAGAGTTTGATCCTAGGGGGGGATGAACGCTGGCGGCGTGCCTAATACATGCTTTTCGAGCGAACTGATTAGAAG
CTTGCTTCTATGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGAAGCTAATACCGGATAG
GATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCA
AGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCA
ATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACAAGAGTAACTGCTT
GTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAA
AGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCAC

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.989062
Expected error %
0.400704
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 159 E Value 1e-40 Identity 88.70% Align. Length 334bp (52.02%) Frame 1
Nicotiana tabacum plastid, complete genome
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 92 E Value 6e-20 Identity 84.40% Align. Length 346bp (53.89%) Frame 1
No sequence description found.
E. coli K12 Genome (E.coli_K12/Ecoli_genome)NCBI BLAST
gi|16127994|ref|NC_000913.1| Score 147 E Value 1e-35 Identity 86.80% Align. Length 366bp (57.01%) Frame 1
Escherichia coli K12, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0238M13
Clone
Clone name
hba-238m13
LE_HBa0238M13
P238M13
Clone type
BAC
Contamination
none
Estimated length
81,222
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found