Clone Read LE_HBa0242H19_T7_404129

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0242H19_T7_404129
Primer
T7
ID from Seq. Facility
LE_HBa_242_H19-T7
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
794 trimmed for vector and quality, 854 raw
Sequence
>LE_HBa0242H19_T7_404129 (raw untrimmed)
GGGGTCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAATGAGTCATTCTTCTTAGGGGAGGGATGGTGTCTTGTCTTGAGGTATCAAGGGAGGTTGTATG
TTCCTAATGTCGATGGTTTGAGGAATCGGATTCTTGAGGAAGCTCATGAGTCCCGTTACTCCATTTATCCGAGTTCTACAAAGATGTACCATGACCTTAG
GGAAGTGTTTTGGTGGGAATACTTGAAGAAGGACATAGCGGAATTTGTTGCAAAGTTTCCAAATTGCCAATAAGTGAAAGTCAAACACCAAAATCTAGGT
GGTTTACTTAAAGAAATCCCAATACCTACTTAGAAATGGGAAGACATCAATATGGATTTTGTGGTAGGTTTTCCTCGGACACAAAATCAATATGAATCCA
TGTGGGTGGTTGTGGATAGGTTGATTGAATCCTCTCATTTTATTCCCGTCAAGTTACTTATTCGGCAAAGGATTACGCAAGGATCTTCATAGATGAGATT
GTGTGTTGCCATGGCATTCCATTATCGATCATATCGGACAGGGGTGCACAATTCACAGCTAGATTTTGGAGGTCATTCCAAAATGGATGGGTACCAAGGT
GAAGCTAAGTAGTAGTTTTCATCCTCAAACGGATGGTCAAGTAGAGCATACTATTCAAACCTGTTAGGATATTCTTAGAGCTTATATAATTGATTCTAAG
GGGAATTGGGATAAACACTTGCCTCTAGTGGAGTTTTCTTACAATAATAGCTACCATTCATGTCATGACCCACAACGAGCCGCGAGTGGCACCCACACTT
ATCCTACTATGTGAGCGAACCAACCAATCTAAACCCCAACATTTCAAACATAAA
>LE_HBa0242H19_T7_404129 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTAATGAGTCATTCTTCTTAGGGGAGGGATGGTGTCTTGTCTTGAGGTATCAAGGGAGGTTGTATGTTCCT
AATGTCGATGGTTTGAGGAATCGGATTCTTGAGGAAGCTCATGAGTCCCGTTACTCCATTTATCCGAGTTCTACAAAGATGTACCATGACCTTAGGGAAG
TGTTTTGGTGGGAATACTTGAAGAAGGACATAGCGGAATTTGTTGCAAAGTTTCCAAATTGCCAATAAGTGAAAGTCAAACACCAAAATCTAGGTGGTTT
ACTTAAAGAAATCCCAATACCTACTTAGAAATGGGAAGACATCAATATGGATTTTGTGGTAGGTTTTCCTCGGACACAAAATCAATATGAATCCATGTGG
GTGGTTGTGGATAGGTTGATTGAATCCTCTCATTTTATTCCCGTCAAGTTACTTATTCGGCAAAGGATTACGCAAGGATCTTCATAGATGAGATTGTGTG
TTGCCATGGCATTCCATTATCGATCATATCGGACAGGGGTGCACAATTCACAGCTAGATTTTGGAGGTCATTCCAAAATGGATGGGTACCAAGGTGAAGC
TAAGTAGTAGTTTTCATCCTCAAACGGATGGTCAAGTAGAGCATACTATTCAAACCTGTTAGGATATTCTTAGAGCTTATATAATTGATTCTAAGGGGAA
TTGGGATAAACACTTGCCTCTAGTGGAGTTTTCTTACAATAATAGCTACCATTCATGTCATGACCCACAACGAGCCGCGAGTGGCACCCACACT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.972065
Expected error %
0.141186
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L38948.1:25-452 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (3.90%) Frame 2
Cloning vector pKIL128 ccdB gene, complete cds
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (3.90%) Frame 2
pT7T3D cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0242H19
Clone
Clone name
hba-242h19
LE_HBa0242H19
C06HBa0242H19
P242H19
Clone type
BAC
Contamination
none
Estimated length
98,250
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found