Clone Read LE_HBa0230K14_SP6_405571

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0230K14_SP6_405571
Primer
SP6
ID from Seq. Facility
LE_HBa_230_K14-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
2 trimmed for vector and quality, 1844 raw
Sequence
>LE_HBa0230K14_SP6_405571 (raw untrimmed)
AGATCTCTAGTTCGCTAAATCTAGGACTGAACCTGTATTCTACAACTTAGTATTACGACTTTGGAAACAGATATCCTACCTCTCACCCCGTAGCGCACCG
TCATAAATAGTTTGCCGANAATGAGATGGAATGATATACGTGCTTTTACGATGACCTCTCGTCGCGCTACATGGGATCTATAGAGTGATGTTAGCAATAT
ACACTATATATGCACAGAGTATACAATCTGGACGTTATCGCACTCATCGTTAAGTACCAGACTGCTGTTATTGCTATGTTCTGGCGCAGAGTAATTGCAT
TTCACTACTCGGCGCCGTGGCGTGTTGAGTGCGAGCGTCTCGATCAGAATATGATAATACTACTCTATACTGATTGAGCTTGGATCTATATACATAGCGT
ATATGTACTATATATAGTCGTACAACTGGCGTACGCCCCCATATGAATGACTGCTGATCATAATCGAGGTTGTCTGACTTATGTATGGGTACGACGTATT
GATAGCATGAGTACATATTAGCGTGACAGTTCAGTATCTTCTGTCGCTTTGACGATAGATTGTACCTCGTGTACATGCGCATCGAGGAGAATCTCTACGT
AGACCATATAGATGTCGAGTGATGCGGATAGATGTATCGATGATCTAGGCCTCAATGTGGATCGCTTATGTGCGATACAGCTTATATATGCAGTATTGCT
AATTCGTGTACATATATGAACGTAGAATACTAGCATGTAAATAGTCTGTTCGTACAGACGAGTCTTTGAGTCGACATTCTCCGCTTATACCTGTGAAACC
TGATTTATCTAGTGTGCTCTGTATGGACGTATTGATAGCTTGGTGCTGAATTAGTCTAGATGCAGAAGGCGCGAGAAATCACCCGACTCCTTCACTCATG
CGTCAGATCTCAACAGATGAAGTATACCTGCTGTCTGTACTACTGTAGATGCACTCTTGACGAGCTAAGACAGGCTGATGAATAATTGCATAGACCGCAA
TTATTGTCGCTGCGAAGTCCTAGCTGAGACTGTGCCAAGCTTGATCAGCAGTTCTGATACCGAGCACGCTTAGCGCCGGCTCAATGTAGAGTGCTCGGAA
ACGAGCTATATGAGGTTATGCTAGCGTAACTGTCTGATGCACACTGAAGGTGAATTAAACTGCTGGAATTGCCATAGCATTTGTAGACGATATTTAATCT
ACAGTAGTTGATTATCTTGATCTTCATGTAGATCTATGCGCGAACACATACTCATCTATATGTGCCGATGACTGCACTGTACGTCGACATCGTATCAGTA
TCACACAGATGCTCGATAAGCTGCCCAGATGCCCTGAACTTTGTCTAGAGATGGATGGGGTGCTCGATGATACCGCATGTCACTTAGTTGTTGTTTCACC
TCCTCTTGCCATACGTCAGTAGCAGAGTTATAATACCTGAATGATCAGATTGTCGGTGGAATGACGTATGCAGATGATGTCACTGCCATACTCGATTACT
CTACTCTTAATCTGCATACATTGGAATATCTGCTGCTGTTGTAAATGGAGACAAGTCAGGTCAGCTGAATGCTGTGATAGATCCATGTAATACAGTACTT
GTNTGAGATGTATTTATTCGTACTCGATCGATATCCTTGCTACTGACCTCATCTGTGCAGCATTCTGACGTGTAAGTGCTTGAATCTACCTCTTACATGT
GCCACTGGGCTCCTCTCGACGATGCTCGCGAGTTACTCACTGGCGAACAGTAGATACTAAGCAAGAACTGCTTCAGTACAACGTAGAAGCGTCTCGAGCC
TGGAGATACACGGTGAAGTGTACACTCCTGCTTGTACACATCGA
>LE_HBa0230K14_SP6_405571 (trimmed for quality and cloning vector)
GT

This is probably not a useful sequence.

Problems:
  • High predicted error rate
  • Short cloning insert
  • Low complexity
 Warnings:
  • Not screened for repetitive elements
  • Not screened for contaminants
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
4.28712
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0230K14
Clone
Clone name
hba-230k14
LE_HBa0230K14
P230K14
Clone type
BAC
Contamination
none
Estimated length
106,964
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found