Clone Read LE_HBa0009K06_T7_4331

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0009K06_T7_4331
Primer
T7
ID from Seq. Facility
LE_HBa_009_K06-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
705 trimmed for vector and quality, 924 raw
Genbank Accession
DU024296
Sequence
>LE_HBa0009K06_T7_4331 (raw untrimmed)
AACGGGATCCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATATACTATATGAAGAAGAAATTCCTTTATTATTTTATTGTGTCTCGATTT
ATATTATGGAATTCCCTTCCTGCTACATGTTTGCCTGAAAGCTTTAGTTGATTTTTCTGATGTTAATTCAACTTTTTTGGCAGGACTCCAAAGCACAGGT
GACTGCAATACACTTGATAATAGGAACTCTTCAGAGAATGCACATCTTTGGTGTTGAGAACAGGGACACGTTAACGCACAAGGCTACAGGGGTAATTATC
TTAGCATCTCCTATTTCCTTTTGCTGAAGTTAATCTATTACAAATTAATTATAATCACTGTTGCCCTTTTCACCCTCCAGTACTCTGCAAAGCTCTTGAA
GAAACCTGATCAATGTAGAGCTGTGTATGCTTGTTCGCATCTTTTCTGGGTTGACGATCAGGACAATATCAAGGATGGAGAAAGGTTCATAGCTGTGCCT
TGCGTGTTTAGGGGATAAAATATACATTCAAATATGCTATGTCTCTTTGGAGTTCCTTTTAGTTTCATGCTTTTCTGTAGCCATCTTCTTATGTGTTACT
TATTTCCCCTATTGATTCAGGGTTTTGCTTTGCTTAAAGCGGGCCTTAAGAATTGCAAATGCTGCTCAACAAATGTCCAATGCAACCCGAGGCAGCAGTG
GATCTGTCTTGCTCTTTATAGAAATTCTAAACAAGTAAAACCAACTCTTCATTCCTGGTCTTTTTACCTTCTCAACTACTGTTTCTTTTGGAAGTAATTT
TACAAGAAAATTCTTTTGTATTAAAAGGTATCTGTATTTCTTTGAGAAAGGGGGGTCTCACAAATTATATGGTTTGCTCCCGTCCCCAAGCCCGGATTGG
ACCTTATATCCCAACTGGAAATGC
>LE_HBa0009K06_T7_4331 (trimmed for quality and cloning vector)
AGCTTATATACTATATGAAGAAGAAATTCCTTTATTATTTTATTGTGTCTCGATTTATATTATGGAATTCCCTTCCTGCTACATGTTTGCCTGAAAGCTT
TAGTTGATTTTTCTGATGTTAATTCAACTTTTTTGGCAGGACTCCAAAGCACAGGTGACTGCAATACACTTGATAATAGGAACTCTTCAGAGAATGCACA
TCTTTGGTGTTGAGAACAGGGACACGTTAACGCACAAGGCTACAGGGGTAATTATCTTAGCATCTCCTATTTCCTTTTGCTGAAGTTAATCTATTACAAA
TTAATTATAATCACTGTTGCCCTTTTCACCCTCCAGTACTCTGCAAAGCTCTTGAAGAAACCTGATCAATGTAGAGCTGTGTATGCTTGTTCGCATCTTT
TCTGGGTTGACGATCAGGACAATATCAAGGATGGAGAAAGGTTCATAGCTGTGCCTTGCGTGTTTAGGGGATAAAATATACATTCAAATATGCTATGTCT
CTTTGGAGTTCCTTTTAGTTTCATGCTTTTCTGTAGCCATCTTCTTATGTGTTACTTATTTCCCCTATTGATTCAGGGTTTTGCTTTGCTTAAAGCGGGC
CTTAAGAATTGCAAATGCTGCTCAACAAATGTCCAATGCAACCCGAGGCAGCAGTGGATCTGTCTTGCTCTTTATAGAAATTCTAAACAAGTAAAACCAA
CTCTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.959123
Expected error %
0.399805
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g75850.1 Score 74 E Value 9e-29 Identity 86.70% Align. Length 551bp (78.16%) Frame 3
68414.m08810 vacuolar protein sorting-associated protein 35 family protein / VPS35 family protein similar to vacuolar protein sorting 35 [Mus musculus] GI:11875394; contains Pfam profile PF03635: Vacuolar protein sorting-associated protein 35
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g17790.1 Score 69 E Value 1e-26 Identity 84.00% Align. Length 551bp (78.16%) Frame 3
68415.m02062 vacuolar protein sorting-associated protein 35 family protein / VPS35 family protein similar to vacuolar protein sorting 35 [Mus musculus] GI:11875394; contains Pfam profile PF03635: Vacuolar protein sorting-associated protein 35
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_469973.1| Score 75 E Value 3e-27 Identity 92.00% Align. Length 551bp (78.16%) Frame 3
putative vacuolar protein sorting-associated protein [Oryza sativa (japonica cultivar-group)] gb|AAO72382.1| putative vacuolar protein sorting-associated protein [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF26771.2| Score 74 E Value 4e-27 Identity 86.50% Align. Length 307bp (43.55%) Frame 3
T4O12.9 [Arabidopsis thaliana] pir||G96787 protein T4O12.9 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0009K06
Clone
Clone name
hba-9k6
LE_HBa0009K06
C02HBa0009K06
P009K06
Clone type
BAC
Contamination
none
Estimated length
158,936
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found