Clone Read LE_HBa0053B23_SP6_43484

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0053B23_SP6_43484
Primer
SP6
ID from Seq. Facility
LE_HBa_053_B23-SP6
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
33 trimmed for vector and quality, 867 raw
Sequence
>LE_HBa0053B23_SP6_43484 (raw untrimmed)
CTAGTGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNCNNNNANCCCCNTTGNNNAANGANNACNANCNACNNCCCAAN
CCCTCACTCTCCCAAGANGANNGANNGANAANGAGACNAGGGCCCAGNACNCCAAGCCGAGTCNCANGNCGACCCACNTCCACCGGACANCAANNCAGNN
NGNNNGAGCCNCAGGANNNNGNCNNAGGGGNNNGAGCCCCAGGAANNNNNNCNCNNGAGCAAGCNNAAAACACANAAACAAACCNGNACNCGCAACCAAA
AACCCACNAGACCAACCCACAACGACCAAAGAAAACCACAGCCAAACAACAAGAANAGANGAGCGAGCAAGACCCAACANCAAGCAGAACGCACAAGCAC
AAAACCACACCCCAAGAAAACGGACGGNAGCCAGACANAAACAAGAGGCAGAAACACACAACAAAGCGAGCGCGCACCCAAGGGGGNACGAACANCCNAG
NCNNAA
ACAGCCCAAGGAGGAAGAAANCAAAAGGCCAAAGACCCAAAACGCAGAGCCGAAACCCCAGGGAGCGGCACGCCCGCCCGCAAAACCCCCCAAA
ACAAACAGCGCAAAAGACCACAAAAAGNACCGGAAAAAAGGGACAAGCACCCCGGCCGAACAACAAAAGCCCAACACGCAAGGCAAAACACCCCACCAAC
GACAACAGCGCGCACACAAAAAGGGCCACCGAAGGCNGCACCCCGCAGAAACAGCGAAAAAACACCCAACCCCCCACGGAGACCAAAGCACCACCAACGC
ACGGCCAAGCGACCCCGGGGCACGGACAAACCAGCAAAGGCACAAGNCGCAAAAACACAAAGAAGCG
>LE_HBa0053B23_SP6_43484 (trimmed for quality and cloning vector)
ACAGCCCAAGGAGGAAGAAANCAAAAGGCCAAA

This is probably not a useful sequence.

Problems:
  • Low complexity
  • High predicted error rate
  • Short cloning insert
Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.693599
Expected error %
6.50264
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0053B23
Clone
Clone name
hba-53b23
LE_HBa0053B23
C04HBa0053B23
P053B23
Clone type
BAC
Contamination
none
Estimated length
159,087
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found