Clone Read LE_HBa0010A10_SP6_4626

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0010A10_SP6_4626
Primer
SP6
ID from Seq. Facility
LE_HBa_010_A10-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
658 trimmed for vector and quality, 971 raw
Genbank Accession
DU024532
Sequence
>LE_HBa0010A10_SP6_4626 (raw untrimmed)
CGTTTAGGCGGACGCTATAGAAATTACTGGATCAGTCAAAATACGAGGTGCTTTTAAAGGGTAGTTCTCTGAATGAAAAGCAAATGGTAAAGCTTCAGCA
TTTCTGGTGTTACTAAACTAGTTTTAAGATAAGCAGGGTCACATTGTTACCTGAGGAAAATGCTTTGATGCGTCCACTATCAAGACTGGTCTTCCATTGA
CATCAAAACTGTCATGCACATATGCTTTTCCAGTTTCAGCACTATTTTTAACTGACTCTTCTGACAAGTCTGATACACCAAATTCATGACGCCATCTCTG
ATATGAAAGTTTGGGAGATACATCATTGTAAGTGTCCCTTCTTACTCTTGAAGCTTACAGTAACTATTATAGCATTGTAAATATTCAGCTCAGATGCAGC
TCTTAAGTAATTTCATGATAATTGAAATGAAGGATTGTCACAGCAAAACAAGTCTACTGGCAGGAAGGAGGAAAAATACTCCCATTCTTCTCATAACCAT
GAGGAAATGAAATGTTTGTAGTAATCCTAGGCTCTTAGCAATACAGTGTCCCATGTTTTATATAGCCACTTCATGAGAAATTCTTTTGACACAGTGTGGA
CAAGTCAATGAAAGGCAGAGGAAGAATAAATCTATTCTCTCCACCTTCAGAAACCAGATGGAAGGAGGAAAATAAATCAGAGAAGAGTAAAAAGAAGAAG
ATAGCTGATGCCTTATGGGTGAAGATATGATGATGTGCTAATCAGCATTAGTAGAGCTACATTTTATTTTCTTTTAAATAAAAAAGTCAGAGTATGACAG
TCAAATTATCGTGAAGATTCAAGTAACATAAACCACATAATTTACATGGGCTTATGCACCTTGGAAACCACATCCTCAACTGAAAATTCCGATCCTTCAG
AACCATAGATCTTTTCTTCTCGTCCCTTCATTTCTCCAACGGAAATCTGAATTCCCCTTTAATCTCTCTTA
>LE_HBa0010A10_SP6_4626 (trimmed for quality and cloning vector)
GTTCTCTGAATGAAAAGCAAATGGTAAAGCTTCAGCATTTCTGGTGTTACTAAACTAGTTTTAAGATAAGCAGGGTCACATTGTTACCTGAGGAAAATGC
TTTGATGCGTCCACTATCAAGACTGGTCTTCCATTGACATCAAAACTGTCATGCACATATGCTTTTCCAGTTTCAGCACTATTTTTAACTGACTCTTCTG
ACAAGTCTGATACACCAAATTCATGACGCCATCTCTGATATGAAAGTTTGGGAGATACATCATTGTAAGTGTCCCTTCTTACTCTTGAAGCTTACAGTAA
CTATTATAGCATTGTAAATATTCAGCTCAGATGCAGCTCTTAAGTAATTTCATGATAATTGAAATGAAGGATTGTCACAGCAAAACAAGTCTACTGGCAG
GAAGGAGGAAAAATACTCCCATTCTTCTCATAACCATGAGGAAATGAAATGTTTGTAGTAATCCTAGGCTCTTAGCAATACAGTGTCCCATGTTTTATAT
AGCCACTTCATGAGAAATTCTTTTGACACAGTGTGGACAAGTCAATGAAAGGCAGAGGAAGAATAAATCTATTCTCTCCACCTTCAGAAACCAGATGGAA
GGAGGAAAATAAATCAGAGAAGAGTAAAAAGAAGAAGATAGCTGATGCCTTATGGGTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.961796
Expected error %
0.389717
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD28849.1| Score 77 E Value 3e-13 Identity 24.30% Align. Length 143bp (21.73%) Frame 2
SEC14 cytosolic factor-like [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0010A10
Clone
Clone name
hba-10a10
LE_HBa0010A10
C12HBa0010A10
P010A10
Clone type
BAC
Contamination
none
Estimated length
97,786
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found